Determination of ketorolac in human plasma by reversed-phase high-performance liquid chromatography using solid-phase extraction and ultraviolet detection

Zhao Wang; Richard M. Dsida; Michael J. Avram

doi:10.1016/S0378-4347(01)00134-7

Determination of ketorolac in human plasma by reversed-phase high-performance liquid chromatography using solid-phase extraction and ultraviolet detection

Zhao Wang, Richard M. Dsida, Michael J. Avram^*

^*Corresponding author for this work

Anesthesiology

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

An improved high-performance liquid chromatographic method has been developed to measure human plasma concentrations of the analgesic nonsteroidal anti-inflammatory drug ketorolac for use in pharmacokinetic studies. Samples were prepared for analysis by solid-phase extraction using Bond-Elut PH columns, with nearly complete recovery of both ketorolac and the internal standard tolmetin. The two compounds were separated on a Radial-Pak C₁₈ column using a mobile phase consisting of water-acetonitrile-1.0 mol/l dibutylamine phosphate (pH 2.5) (30:20:1) and detected at a UV wavelength of 313 nm. Using only 250 μl of plasma, the standard curve was linear from 0.05 to 10.0 μg/ml.

Original language	English (US)
Pages (from-to)	383-386
Number of pages	4
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	755
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-4347(01)00134-7
State	Published - May 5 2001

Keywords

Ketorolac

ASJC Scopus subject areas

Chemistry(all)

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10.1016/S0378-4347(01)00134-7

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title = "Determination of ketorolac in human plasma by reversed-phase high-performance liquid chromatography using solid-phase extraction and ultraviolet detection",

abstract = "An improved high-performance liquid chromatographic method has been developed to measure human plasma concentrations of the analgesic nonsteroidal anti-inflammatory drug ketorolac for use in pharmacokinetic studies. Samples were prepared for analysis by solid-phase extraction using Bond-Elut PH columns, with nearly complete recovery of both ketorolac and the internal standard tolmetin. The two compounds were separated on a Radial-Pak C18 column using a mobile phase consisting of water-acetonitrile-1.0 mol/l dibutylamine phosphate (pH 2.5) (30:20:1) and detected at a UV wavelength of 313 nm. Using only 250 μl of plasma, the standard curve was linear from 0.05 to 10.0 μg/ml.",

keywords = "Ketorolac",

author = "Zhao Wang and Dsida, {Richard M.} and Avram, {Michael J.}",

note = "Funding Information: Supported in part by a grant from Hoffman-La Roche, Nutley, NJ, USA.",

year = "2001",

month = may,

day = "5",

doi = "10.1016/S0378-4347(01)00134-7",

language = "English (US)",

volume = "755",

pages = "383--386",

journal = "Journal of Chromatography B: Biomedical Sciences and Applications",

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publisher = "Elsevier BV",

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}

Determination of ketorolac in human plasma by reversed-phase high-performance liquid chromatography using solid-phase extraction and ultraviolet detection. / Wang, Zhao; Dsida, Richard M.; Avram, Michael J.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 755, No. 1-2, 05.05.2001, p. 383-386.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Determination of ketorolac in human plasma by reversed-phase high-performance liquid chromatography using solid-phase extraction and ultraviolet detection

AU - Wang, Zhao

AU - Dsida, Richard M.

AU - Avram, Michael J.

N1 - Funding Information: Supported in part by a grant from Hoffman-La Roche, Nutley, NJ, USA.

PY - 2001/5/5

Y1 - 2001/5/5

N2 - An improved high-performance liquid chromatographic method has been developed to measure human plasma concentrations of the analgesic nonsteroidal anti-inflammatory drug ketorolac for use in pharmacokinetic studies. Samples were prepared for analysis by solid-phase extraction using Bond-Elut PH columns, with nearly complete recovery of both ketorolac and the internal standard tolmetin. The two compounds were separated on a Radial-Pak C18 column using a mobile phase consisting of water-acetonitrile-1.0 mol/l dibutylamine phosphate (pH 2.5) (30:20:1) and detected at a UV wavelength of 313 nm. Using only 250 μl of plasma, the standard curve was linear from 0.05 to 10.0 μg/ml.

AB - An improved high-performance liquid chromatographic method has been developed to measure human plasma concentrations of the analgesic nonsteroidal anti-inflammatory drug ketorolac for use in pharmacokinetic studies. Samples were prepared for analysis by solid-phase extraction using Bond-Elut PH columns, with nearly complete recovery of both ketorolac and the internal standard tolmetin. The two compounds were separated on a Radial-Pak C18 column using a mobile phase consisting of water-acetonitrile-1.0 mol/l dibutylamine phosphate (pH 2.5) (30:20:1) and detected at a UV wavelength of 313 nm. Using only 250 μl of plasma, the standard curve was linear from 0.05 to 10.0 μg/ml.

KW - Ketorolac

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IS - 1-2

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Determination of ketorolac in human plasma by reversed-phase high-performance liquid chromatography using solid-phase extraction and ultraviolet detection

Abstract

Keywords

ASJC Scopus subject areas

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