Abstract
A sensitive chemiluminescence method has been proposed for Phenobarbital (PB) determination. It is based on the enhancive effect of PB on the chemiluminescence reaction between luminol and dissolved oxygen in a flow injection system. The chemiluminescence intensity linearly responded to the PB concentrations ranged from 0.05 to 10 ng/ml with the detection limit of 0.02 ng/ml (3σ). At a flow rate of 2.0 ml/ min, the whole procedure of PB determination (including sampling and washing) takes just 0.5 minute, offering the sampling efficiency of 120 per 1 h. The method was applied successfully for the PB assay in pharmaceutical preparations, human urine and serum without any pretreatment with recovery from 95.7 to 106.7% and RSDs of less than 3.0%.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 184-187 |
| Number of pages | 4 |
| Journal | Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry |
| Volume | 5 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 2011 |
Funding
ACKNOWLEDGMENTS The authors gratefully acknowledge the financial support from Shaanxi Province Nature Science Foun dation and Ministry of Education, China, Grant No. 2006B05 and No. 07JK395 and the NWU Grad uate Innovation and Creativety Funds and NWU Graduate Experimental Research Funds, China, grant nos. 09YZZ45 and 09YSY18.
Keywords
- Chemiluminescence
- Luminel
- Phenobarbital
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Clinical Biochemistry