Determination of phenobarbital in human urine and serum using flow injection chemiluminescence

X. Li, L. C. Niu, X. L. He, Z. H. Song*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


A sensitive chemiluminescence method has been proposed for Phenobarbital (PB) determination. It is based on the enhancive effect of PB on the chemiluminescence reaction between luminol and dissolved oxygen in a flow injection system. The chemiluminescence intensity linearly responded to the PB concentrations ranged from 0.05 to 10 ng/ml with the detection limit of 0.02 ng/ml (3σ). At a flow rate of 2.0 ml/ min, the whole procedure of PB determination (including sampling and washing) takes just 0.5 minute, offering the sampling efficiency of 120 per 1 h. The method was applied successfully for the PB assay in pharmaceutical preparations, human urine and serum without any pretreatment with recovery from 95.7 to 106.7% and RSDs of less than 3.0%.

Original languageEnglish (US)
Pages (from-to)184-187
Number of pages4
JournalBiochemistry (Moscow) Supplement Series B: Biomedical Chemistry
Issue number2
StatePublished - Jun 1 2011


  • Chemiluminescence
  • Luminel
  • Phenobarbital

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Clinical Biochemistry

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