A sensitive chemiluminescence method has been proposed for Phenobarbital (PB) determination. It is based on the enhancive effect of PB on the chemiluminescence reaction between luminol and dissolved oxygen in a flow injection system. The chemiluminescence intensity linearly responded to the PB concentrations ranged from 0.05 to 10 ng/ml with the detection limit of 0.02 ng/ml (3σ). At a flow rate of 2.0 ml/ min, the whole procedure of PB determination (including sampling and washing) takes just 0.5 minute, offering the sampling efficiency of 120 per 1 h. The method was applied successfully for the PB assay in pharmaceutical preparations, human urine and serum without any pretreatment with recovery from 95.7 to 106.7% and RSDs of less than 3.0%.
|Original language||English (US)|
|Number of pages||4|
|Journal||Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry|
|State||Published - Jun 1 2011|
ASJC Scopus subject areas
- Molecular Medicine
- Clinical Biochemistry