Determination of phenobarbital in human urine and serum using flow injection chemiluminescence

X. Li*, L. C. Niu, X. L. He, Z. H. Song

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


A sensitive chemiluminescence method, based on the enhancive effect of phenobarbital on the chemiluminescence reaction between luminol and dissolved oxygen in a flow injection system, was proposed for the determination of phenobarbital. The chemiluminescencc intensity responded to the concentration of phenobarbital linearly ranging from 0.05 to 10 ng·ml-1 with the detection limit of 0.02 ng·ml-1 (3σ). At a flow rate of 2.0 ml·min-1, a complete determination of phenobarbital, including sampling and washing, could be accomplished in 0.5 min, offering the sampling efficiency of 120 h-1 accordingly. The method was applied successfully in an assay of PB for pharmaceutical preparations, human urine and scrum without any pretreatment with recovery from 95.7 to 106.7% and RSDs of less than 3.0%.

Original languageEnglish (US)
Pages (from-to)88-94
Number of pages7
JournalBiomeditsinskaya Khimiya
Issue number1
StatePublished - 2012


  • Chemiluminescence
  • Luminol
  • Phenobarbital

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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