TY - JOUR
T1 - Deuterosome-Mediated Centriole Biogenesis
AU - KlosDehring, Deborah A.
AU - Vladar, Eszter K.
AU - Werner, Michael E.
AU - Mitchell, Jennifer W.
AU - Hwang, Peter
AU - Mitchell, Brian J.
N1 - Funding Information:
This work was supported by the NIH/NIGMS (R01GM089970 to B.J.M.) and by a pilot grant from the NU-SDRC NIH/NIAMS (5P30AR057216-05). D.A.K.D. was supported by an ARRA NRSA grant (1F32AI08006901A1), and M.E.W. was supported by a postdoctoral fellowship from the American Heart Association.
PY - 2013/10/14
Y1 - 2013/10/14
N2 - The ability of cells to faithfully duplicate their two centrioles once per cell cycle is critical for proper mitotic progression and chromosome segregation. Multiciliated cells represent an interesting variation of centriole duplication in that these cells generate greater than 100 centrioles, which form the basal bodies of their motile cilia. This centriole amplification is proposed to require a structure termed the deuterosome, thought to be capable of promoting de novo centriole biogenesis. Here, we begin to molecularly characterize the deuterosome and identify it as a site for the localization of Cep152, Plk4, andSAS6. Additionally we identify CCDC78 as a centriole-associated and deuterosome protein that is essential for centriole amplification. Overexpression of Cep152, but not Plk4, SAS6, or CCDC78, drives overamplification of centrioles. However, in CCDC78 morphants, Cep152 fails to localize to the deuterosome and centriole biogenesis is impaired, indicating that CCDC78-mediated recruitment of Cep152 is required for deuterosome-mediated centriole biogenesis.
AB - The ability of cells to faithfully duplicate their two centrioles once per cell cycle is critical for proper mitotic progression and chromosome segregation. Multiciliated cells represent an interesting variation of centriole duplication in that these cells generate greater than 100 centrioles, which form the basal bodies of their motile cilia. This centriole amplification is proposed to require a structure termed the deuterosome, thought to be capable of promoting de novo centriole biogenesis. Here, we begin to molecularly characterize the deuterosome and identify it as a site for the localization of Cep152, Plk4, andSAS6. Additionally we identify CCDC78 as a centriole-associated and deuterosome protein that is essential for centriole amplification. Overexpression of Cep152, but not Plk4, SAS6, or CCDC78, drives overamplification of centrioles. However, in CCDC78 morphants, Cep152 fails to localize to the deuterosome and centriole biogenesis is impaired, indicating that CCDC78-mediated recruitment of Cep152 is required for deuterosome-mediated centriole biogenesis.
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U2 - 10.1016/j.devcel.2013.08.021
DO - 10.1016/j.devcel.2013.08.021
M3 - Article
C2 - 24075808
AN - SCOPUS:84885390501
SN - 1534-5807
VL - 27
SP - 103
EP - 112
JO - Developmental Cell
JF - Developmental Cell
IS - 1
ER -