Deuterosome-Mediated Centriole Biogenesis

Deborah A. KlosDehring, Eszter K. Vladar, Michael E. Werner, Jennifer W. Mitchell, Peter Hwang, Brian J. Mitchell*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

103 Scopus citations


The ability of cells to faithfully duplicate their two centrioles once per cell cycle is critical for proper mitotic progression and chromosome segregation. Multiciliated cells represent an interesting variation of centriole duplication in that these cells generate greater than 100 centrioles, which form the basal bodies of their motile cilia. This centriole amplification is proposed to require a structure termed the deuterosome, thought to be capable of promoting de novo centriole biogenesis. Here, we begin to molecularly characterize the deuterosome and identify it as a site for the localization of Cep152, Plk4, andSAS6. Additionally we identify CCDC78 as a centriole-associated and deuterosome protein that is essential for centriole amplification. Overexpression of Cep152, but not Plk4, SAS6, or CCDC78, drives overamplification of centrioles. However, in CCDC78 morphants, Cep152 fails to localize to the deuterosome and centriole biogenesis is impaired, indicating that CCDC78-mediated recruitment of Cep152 is required for deuterosome-mediated centriole biogenesis.

Original languageEnglish (US)
Pages (from-to)103-112
Number of pages10
JournalDevelopmental Cell
Issue number1
StatePublished - Oct 14 2013

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology


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