Developing top down proteomics to maximize proteome and sequence coverage from cells and tissues

Dorothy R. Ahlf, Paul M. Thomas, Neil L. Kelleher*

*Corresponding author for this work

Research output: Contribution to journalReview article

36 Scopus citations

Abstract

Mass spectrometry based proteomics generally seeks to identify and characterize protein molecules with high accuracy and throughput. Recent speed and quality improvements to the independent steps of integrated platforms have removed many limitations to the robust implementation of top down proteomics (TDP) for proteins below 70. kDa. Improved intact protein separations coupled to high-performance instruments have increased the quality and number of protein and proteoform identifications. To date, TDP applications have shown >1000 protein identifications, expanding to an average of ~3-4 more proteoforms for each protein detected. In the near future, increased fractionation power, new mass spectrometers and improvements in proteoform scoring will combine to accelerate the application and impact of TDP to this century's biomedical problems.

Original languageEnglish (US)
Pages (from-to)787-794
Number of pages8
JournalCurrent Opinion in Chemical Biology
Volume17
Issue number5
DOIs
StatePublished - Oct 1 2013

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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