Development of a new tool for 3D modeling for regenerative medicine

Filippo Mattoli, Roberto Tiribuzi, Francesco D'Angelo, Ilaria Di Girolamo, Mattia Quattrocelli, Simona Montesano, Lucia Crispoltoni, Vasileios Oikonomou, Maria Gabriella Cusella De Angelis, Peggy Marconi, Antonio Orlacchio, Maurilio Sampaolesi, Sabata Martino*, Aldo Orlacchio

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


The effectiveness of therapeutic treatment based on regenerative medicine for degenerative diseases (i.e., neurodegenerative or cardiac diseases) requires tools allowing the visualization and analysis of the three-dimensional (3D) distribution of target drugs within the tissue. Here, we present a new computational procedure able to overcome the limitations of visual analysis emerging by the examination of a molecular signal within images of serial tissue/organ sections by using the conventional techniques. Together with the 3D anatomical reconstitution of the tissue/organ, our framework allows the detection of signals of different origins (e.g., marked generic molecules, colorimetric, or fluorimetric substrates for enzymes; microRNA; recombinant protein). Remarkably, the application does not require the employment of specific tracking reagents for the imaging analysis. We report two different representative applications: the first shows the reconstruction of a 3D model of mouse brain with the analysis of the distribution of the -Galactosidase, the second shows the reconstruction of a 3D mouse heart with the measurement of the cardiac volume.

Original languageEnglish (US)
Article number236854
JournalInternational Journal of Biomedical Imaging
StatePublished - Jan 1 2011

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging


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