Development of a Sensitive Luciferase-Based Sandwich ELISA System for the Detection of Human Extracellular Matrix 1 Protein

Ya Li, Yanqing Li, Junli Zhao, Xiaojing Zheng, Qinwen Mao, Haibin Xia*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Enzyme-linked immunosorbent assay (ELISA) has been one of the main methods for detecting an antigen in an aqueous sample for more than four decades. Nowadays, one of the biggest concerns for ELISA is still how to improve the sensitivity of the assay, and the luciferase-luciferin reaction system has been noticed as a new detection method with high sensitivity. In this study, a luciferin-luciferase reaction system was used as the detection method for a sandwich ELISA system. It was shown that this new system led to an increase in the detection sensitivity of at least two times when compared with the traditional horseradish peroxidase (HRP) detection method. Lastly, the serum levels of the human extracellular matrix 1 protein of breast cancer patients were determined by the new system, which were overall similar to the HRP chemiluminescent system. Furthermore, this new luciferase reporter can be implemented into other ELISA systems for the purpose of increasing the assay sensitivity.

Original languageEnglish (US)
Pages (from-to)273-279
Number of pages7
JournalMonoclonal Antibodies in Immunodiagnosis and Immunotherapy
Volume35
Issue number6
DOIs
StatePublished - Dec 2016

Keywords

  • extracellular matrix 1
  • horseradish peroxidase
  • luciferase
  • monoclonal antibody
  • sandwich ELISA

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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