Abstract
Enzyme-linked immunosorbent assay (ELISA) has been one of the main methods for detecting an antigen in an aqueous sample for more than four decades. Nowadays, one of the biggest concerns for ELISA is still how to improve the sensitivity of the assay, and the luciferase-luciferin reaction system has been noticed as a new detection method with high sensitivity. In this study, a luciferin-luciferase reaction system was used as the detection method for a sandwich ELISA system. It was shown that this new system led to an increase in the detection sensitivity of at least two times when compared with the traditional horseradish peroxidase (HRP) detection method. Lastly, the serum levels of the human extracellular matrix 1 protein of breast cancer patients were determined by the new system, which were overall similar to the HRP chemiluminescent system. Furthermore, this new luciferase reporter can be implemented into other ELISA systems for the purpose of increasing the assay sensitivity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 273-279 |
| Number of pages | 7 |
| Journal | Monoclonal Antibodies in Immunodiagnosis and Immunotherapy |
| Volume | 35 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 2016 |
Funding
This study was supported by the Innovation Funds of Graduate Programs, Shaanxi Normal University (2013CX B011), and the research grants to H.X. from the National Natural Science Foundation of China (Nos. 81272543 and 81471772) and the Natural Science Foundation of Shaanxi Province, China (No. 2014JM4113).
Keywords
- extracellular matrix 1
- horseradish peroxidase
- luciferase
- monoclonal antibody
- sandwich ELISA
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
