Development of a VX2 Pancreatic Cancer Model in Rabbits: A Pilot Study

Aaron C. Eifler; Robert J. Lewandowski; Sumeet Virmani; Johnathan C. Chung; Dingxin Wang; Richard L. Tang; Barbara Szolc-Kowalska; Gayle E. Woloschak; Guang Yu Yang; Robert K. Ryu; Riad Salem; Andrew C. Larson; Eric Cheon; Matthew Strouch; David J. Bentrem; Reed A. Omary

doi:10.1016/j.jvir.2009.04.051

Development of a VX2 Pancreatic Cancer Model in Rabbits: A Pilot Study

Aaron C. Eifler, Robert J. Lewandowski, Sumeet Virmani, Johnathan C. Chung, Dingxin Wang, Richard L. Tang, Barbara Szolc-Kowalska, Gayle E. Woloschak, Guang Yu Yang, Robert K. Ryu, Riad Salem, Andrew C. Larson, Eric Cheon, Matthew Strouch, David J. Bentrem, Reed A. Omary^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Purpose: An animal model of pancreatic cancer that is large enough to permit imaging and catheterization would be desirable for interventional radiologists to develop novel therapies for pancreatic cancer. The purpose of this study was to test the hypothesis that the VX2 rabbit model of pancreatic cancer could be developed as a suitable platform to test future interventional therapies. Materials and Methods: The authors implanted and grew three pancreatic VX2 tumors per rabbit in six rabbits. Magnetic resonance (MR) imaging was performed at 2 weeks to confirm tumor growth. At 3 weeks, the authors selectively catheterized the gastroduodenal artery under guidance of x-ray digital subtraction angiography (DSA). T2-weighted anatomic imaging, diffusion-weighted MR imaging, and transcatheter intraarterial perfusion (TRIP) MR imaging were then performed. After imaging, tumors were confirmed at necropsy and histopathologically. Tumor sizes at 2 and 3 weeks were compared with a paired t test (P = .05). Results: VX2 pancreatic tumors were grown in all six rabbits. The difference between tumor sizes at 2 and 3 weeks (1.29 cm ± 0.39 vs 1.91 cm ± 0.50, respectively) was significant (P < .001). All tumors were confirmed to be located within pancreatic tissue via histopathologic analysis. DSA and TRIP MR imaging were successful in five rabbits. Diffusion-weighted and anatomic MR imaging were successful in all six rabbits. Conclusions: The VX2 rabbit model of pancreatic cancer is feasible, as verified by imaging and pathologic correlation, and may be a suitable platform to test future interventional therapies.

Original language	English (US)
Pages (from-to)	1075-1082
Number of pages	8
Journal	Journal of Vascular and Interventional Radiology
Volume	20
Issue number	8
DOIs	https://doi.org/10.1016/j.jvir.2009.04.051
State	Published - Aug 2009

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging
Cardiology and Cardiovascular Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jvir.2009.04.051

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Eifler, A. C., Lewandowski, R. J., Virmani, S., Chung, J. C., Wang, D., Tang, R. L., Szolc-Kowalska, B., Woloschak, G. E., Yang, G. Y., Ryu, R. K., Salem, R., Larson, A. C., Cheon, E., Strouch, M., Bentrem, D. J., & Omary, R. A. (2009). Development of a VX2 Pancreatic Cancer Model in Rabbits: A Pilot Study. Journal of Vascular and Interventional Radiology, 20(8), 1075-1082. https://doi.org/10.1016/j.jvir.2009.04.051

@article{2bd1ce97be0f4ac3afbe1d0f31512e55,

title = "Development of a VX2 Pancreatic Cancer Model in Rabbits: A Pilot Study",

abstract = "Purpose: An animal model of pancreatic cancer that is large enough to permit imaging and catheterization would be desirable for interventional radiologists to develop novel therapies for pancreatic cancer. The purpose of this study was to test the hypothesis that the VX2 rabbit model of pancreatic cancer could be developed as a suitable platform to test future interventional therapies. Materials and Methods: The authors implanted and grew three pancreatic VX2 tumors per rabbit in six rabbits. Magnetic resonance (MR) imaging was performed at 2 weeks to confirm tumor growth. At 3 weeks, the authors selectively catheterized the gastroduodenal artery under guidance of x-ray digital subtraction angiography (DSA). T2-weighted anatomic imaging, diffusion-weighted MR imaging, and transcatheter intraarterial perfusion (TRIP) MR imaging were then performed. After imaging, tumors were confirmed at necropsy and histopathologically. Tumor sizes at 2 and 3 weeks were compared with a paired t test (P = .05). Results: VX2 pancreatic tumors were grown in all six rabbits. The difference between tumor sizes at 2 and 3 weeks (1.29 cm ± 0.39 vs 1.91 cm ± 0.50, respectively) was significant (P < .001). All tumors were confirmed to be located within pancreatic tissue via histopathologic analysis. DSA and TRIP MR imaging were successful in five rabbits. Diffusion-weighted and anatomic MR imaging were successful in all six rabbits. Conclusions: The VX2 rabbit model of pancreatic cancer is feasible, as verified by imaging and pathologic correlation, and may be a suitable platform to test future interventional therapies.",

author = "Eifler, {Aaron C.} and Lewandowski, {Robert J.} and Sumeet Virmani and Chung, {Johnathan C.} and Dingxin Wang and Tang, {Richard L.} and Barbara Szolc-Kowalska and Woloschak, {Gayle E.} and Yang, {Guang Yu} and Ryu, {Robert K.} and Riad Salem and Larson, {Andrew C.} and Eric Cheon and Matthew Strouch and Bentrem, {David J.} and Omary, {Reed A.}",

year = "2009",

month = aug,

doi = "10.1016/j.jvir.2009.04.051",

language = "English (US)",

volume = "20",

pages = "1075--1082",

journal = "Journal of Vascular and Interventional Radiology",

issn = "1051-0443",

publisher = "Elsevier Inc.",

number = "8",

}

Eifler, AC, Lewandowski, RJ, Virmani, S, Chung, JC, Wang, D, Tang, RL, Szolc-Kowalska, B, Woloschak, GE , Yang, GY, Ryu, RK, Salem, R, Larson, AC, Cheon, E, Strouch, M, Bentrem, DJ & Omary, RA 2009, 'Development of a VX2 Pancreatic Cancer Model in Rabbits: A Pilot Study', Journal of Vascular and Interventional Radiology, vol. 20, no. 8, pp. 1075-1082. https://doi.org/10.1016/j.jvir.2009.04.051

TY - JOUR

T1 - Development of a VX2 Pancreatic Cancer Model in Rabbits

T2 - A Pilot Study

AU - Eifler, Aaron C.

AU - Lewandowski, Robert J.

AU - Virmani, Sumeet

AU - Chung, Johnathan C.

AU - Wang, Dingxin

AU - Tang, Richard L.

AU - Szolc-Kowalska, Barbara

AU - Woloschak, Gayle E.

AU - Yang, Guang Yu

AU - Ryu, Robert K.

AU - Salem, Riad

AU - Larson, Andrew C.

AU - Cheon, Eric

AU - Strouch, Matthew

AU - Bentrem, David J.

AU - Omary, Reed A.

PY - 2009/8

Y1 - 2009/8

N2 - Purpose: An animal model of pancreatic cancer that is large enough to permit imaging and catheterization would be desirable for interventional radiologists to develop novel therapies for pancreatic cancer. The purpose of this study was to test the hypothesis that the VX2 rabbit model of pancreatic cancer could be developed as a suitable platform to test future interventional therapies. Materials and Methods: The authors implanted and grew three pancreatic VX2 tumors per rabbit in six rabbits. Magnetic resonance (MR) imaging was performed at 2 weeks to confirm tumor growth. At 3 weeks, the authors selectively catheterized the gastroduodenal artery under guidance of x-ray digital subtraction angiography (DSA). T2-weighted anatomic imaging, diffusion-weighted MR imaging, and transcatheter intraarterial perfusion (TRIP) MR imaging were then performed. After imaging, tumors were confirmed at necropsy and histopathologically. Tumor sizes at 2 and 3 weeks were compared with a paired t test (P = .05). Results: VX2 pancreatic tumors were grown in all six rabbits. The difference between tumor sizes at 2 and 3 weeks (1.29 cm ± 0.39 vs 1.91 cm ± 0.50, respectively) was significant (P < .001). All tumors were confirmed to be located within pancreatic tissue via histopathologic analysis. DSA and TRIP MR imaging were successful in five rabbits. Diffusion-weighted and anatomic MR imaging were successful in all six rabbits. Conclusions: The VX2 rabbit model of pancreatic cancer is feasible, as verified by imaging and pathologic correlation, and may be a suitable platform to test future interventional therapies.

AB - Purpose: An animal model of pancreatic cancer that is large enough to permit imaging and catheterization would be desirable for interventional radiologists to develop novel therapies for pancreatic cancer. The purpose of this study was to test the hypothesis that the VX2 rabbit model of pancreatic cancer could be developed as a suitable platform to test future interventional therapies. Materials and Methods: The authors implanted and grew three pancreatic VX2 tumors per rabbit in six rabbits. Magnetic resonance (MR) imaging was performed at 2 weeks to confirm tumor growth. At 3 weeks, the authors selectively catheterized the gastroduodenal artery under guidance of x-ray digital subtraction angiography (DSA). T2-weighted anatomic imaging, diffusion-weighted MR imaging, and transcatheter intraarterial perfusion (TRIP) MR imaging were then performed. After imaging, tumors were confirmed at necropsy and histopathologically. Tumor sizes at 2 and 3 weeks were compared with a paired t test (P = .05). Results: VX2 pancreatic tumors were grown in all six rabbits. The difference between tumor sizes at 2 and 3 weeks (1.29 cm ± 0.39 vs 1.91 cm ± 0.50, respectively) was significant (P < .001). All tumors were confirmed to be located within pancreatic tissue via histopathologic analysis. DSA and TRIP MR imaging were successful in five rabbits. Diffusion-weighted and anatomic MR imaging were successful in all six rabbits. Conclusions: The VX2 rabbit model of pancreatic cancer is feasible, as verified by imaging and pathologic correlation, and may be a suitable platform to test future interventional therapies.

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JO - Journal of Vascular and Interventional Radiology

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Development of a VX2 Pancreatic Cancer Model in Rabbits: A Pilot Study

Abstract

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