Development of microsatellite loci in Artocarpus altilis (Moraceae) and cross-amplification in congeneric species

Colby E Witherup, Diane Ragone, Tyr Wiesner-Hanks, Brian Irish, Brian Scheffler, Sheron Simpson, Francis Zee, M I Zuberi, Nyree JC Zerega

Research output: Contribution to journalArticle

Abstract

Premise of the study: Microsatellite loci were isolated and characterized from enriched genomic libraries of Artocarpus altilis (breadfruit) and tested in four Artocarpus species and one hybrid. The microsatellite markers provide new tools for further studies in Artocarpus . Methods and Results: A total of 25 microsatellite loci were evaluated across four Artocarpus species and one hybrid. Twenty-one microsatellite loci were evaluated on A. altilis (241), A. camansi (34), A. mariannensis (15), and A. altilis × mariannensis (64) samples. Nine of those loci plus four additional loci were evaluated on A. heterophyllus (jackfruit, 426) samples. All loci are polymorphic for at least one species. The average number of alleles ranges from two to nine within taxa. Conclusions: These microsatellite primers will facilitate further studies on the genetic structure and evolutionary and domestication history of Artocarpus species. They will aid in cultivar identification and establishing germplasm conservation strategies for breadfruit and jackfruit.
Original languageEnglish
JournalApplications in Plant Science
Volume1
DOIs
StatePublished - 2013

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