Development of the human/rat chimera model with neonatal rats

Yi Kun Zhang*, Dong Mei Wang, Hong Feng Yuan, Hai Min Li, Ci Xian Bai, Rui Zhang, Lin Chen, Suo Qin Tang, Xue Tao Pei

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


The purpose of this study was to transplant neonatal rat with human cord blood Lin(-) cells to test the possibility of this xenograft model. The Lin(-) cells were purified from human cord blood (CB) using negative selection strategy based on different lineage-specific antigens. The Lin(-) cells were injected into the liver of neonatal rats using a microinjector at an average of 5 x 10(5) cells for each. Peripheral blood (PB) and spleen were collected at 2,4 and 8 weeks after injection. Flow cytometry was performed to detect human cells in the rat PB, PCR was used to detect human cells in PB as well as spleen. The results showed that a definite proportion of human cells existed in peripheral blood of chimeric rat and the human specific beta2 microglobulin gene fragments were detected in spleen genomic DNA of chimeric rat. It is concluded that human/rat chimera model can be developed with neonatal rats. Human/rat xenograft model may provide a useful and convenient method for human hematopoietic stem cell assay in vivo.

Original languageEnglish (US)
Pages (from-to)297-300
Number of pages4
JournalZhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology
Issue number3
StatePublished - Jun 2003
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


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