Development of ultrasound bioprobe for biological imaging

Gajendra S. Shekhawat*, Steven M. Dudek, Vinayak P. Dravid

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

We report the development of an ultrasound bioprobe for in vitro molecular imaging. In this method, the phase of the scattered ultrasound wave is mapped to provide in vitro and intracellular imaging with nanometer-scale resolution under physiological conditions. We demonstrated the technique by successfully imaging a magnetic core in silica core shells and the stiffness image of intracellular fibers in endothelial cells that were stimulated with thrombin. The findings demonstrate a significant advancement in high-resolution ultrasound imaging of biological systems with acoustics under physiological conditions. These will open up various applications in biomedical and molecular imaging with subsurface resolution down to the nanometer scale.

Original languageEnglish (US)
Article numbere1701176
JournalScience Advances
Volume3
Issue number10
DOIs
StatePublished - 2017

Funding

This work used the Scanned Probe Imaging and Development facilities of the NUANCE Center at Northwestern University, which received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource, the Materials Research Science and Engineering Centers (MRSEC) program (NSF DMR-1121262) at the Materials Research Center; the International Institute for Nanotechnology (IIN); the Keck Foundation; and the State of llinois through the IIN. We acknowledge M. Aslam for providing us with silica core shell samples and for constructive discussions. Funding: This study was funded by the SHyNE Resource (NSF ECCS-1542205), the MRSEC program (NSF DMR-1121262), NSF Award No. 1256188, Instrumentation Development for Biological Research (IDBR): Development of Higher Eigenmode Ultrasound Bioprobe for Sub-Cellular Biological Imaging, and the NIH National Heart Lung Blood Institute (grants P01 HL 58064 to S.M.D. and R56 HL HL56088144-06A1 to S.M.D.). Author contributions: G.S. designed and executed the experiments and the feedback circuit development, wrote the paper, and was responsible for Fig. 2. S.M.D. cultured the ECs and transfected them with stimulating agents, provided data analysis, wrote the manuscript, and was responsible for Fig. 3. V.D. provided design of the experiment, edited the paper, performed image analysis, and was responsible for Fig. 1. Competing interests: The authors declare that they have no competing interests. Data and materials availability: All data needed to evaluate the conclusions in the article are present in the article and/or the Supplementary Materials. Additional data related to this paper may be requested from the authors.

ASJC Scopus subject areas

  • General

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