Developmental, malignancy-related, and cross-species analysis of eosinophil, mast cell, and basophil siglec-8 expression

Sherry A. Hudson, Harald Herrmann, Jian Du, Paul Cox, El Bdaoui Haddad, Barbara Butler, Paul R. Crocker, Steven J. Ackerman, Peter Valent, Bruce S. Bochner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Objective The aim of this study is to determine when during hematopoiesis Siglec-8 gets expressed, whether it is expressed on hematologic malignancies, and if there are other non-human species that express Siglec-8. Methods Siglec-8 mRNA and cell surface expression was monitored during in vitro maturation of human eosinophils and mast cells. Flow cytometry was performed on human blood and bone marrow samples, and on blood samples from dogs, baboons, and rhesus and cynomolgus monkeys. Results Siglec-8 is a late maturation marker. It is detectable on eosinophils and basophils from subjects with chronic eosinophilic leukemia, chronic myelogenous leukemia, and on malignant and non-malignant bone marrow mast cells, as well as the HMC-1.2 cell line. None of the Siglec-8 monoclonal antibodies tested recognized leukocytes from dogs, baboons, and rhesus and cynomolgus monkeys. Conclusions Siglec-8-based therapies should not target immature human leukocytes but should recognize mature and malignant eosinophils, mast cells, and basophils. So far, there is no suitable species for preclinical testing of Siglec-8 monoclonal antibodies.

Original languageEnglish (US)
Pages (from-to)1045-1053
Number of pages9
JournalJournal of Clinical Immunology
Volume31
Issue number6
DOIs
StatePublished - Dec 2011

Keywords

  • Basophils
  • Cell lines
  • Eosinophils
  • Expression
  • Hematologic malignancies
  • Mast cells
  • Siglec-8

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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