Developmental regulation of EVF-1, a novel non-coding RNA transcribed upstream of the mouse Dlx6 gene

Jhumku D. Kohtz*, Gord Fishell

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

We previously reported that sonic hedgehog (Shh) induces the differentiation of rat ventral forebrain neurons expressing a novel marker, EVF-1 [Development 125 (1998) 5079]. In this report, we show that EVF-1 is a novel, developmentally regulated, non-coding RNA, with no homology to other known non-coding RNA sequences. Sequence analysis, in vitro translation, and comparison of the rat and mouse EVF-1 sequences suggest that EVF-1 contains no protein coding regions. Chromosomal location indicates that EVF-1 maps adjacent to the Dlx6 gene on mouse chromosome 6. RNA in situ hybridization of the embryonic rat forebrain shows that EVF-1 is expressed by immature neurons in the subventricular zone and its expression decreases during forebrain development. Whole mount in situ hybridization shows that EVF-1 is expressed at high levels in the branchial arches, ventral forebrain, olfactory bulb, and limbs. EVF-1 expression is linked to Shh and the Dlx family of proteins, genes with a demonstrated importance to ventral forebrain and craniofacial development.

Original languageEnglish (US)
Pages (from-to)407-412
Number of pages6
JournalGene Expression Patterns
Volume4
Issue number4
DOIs
StatePublished - Jul 2004

Funding

This work was funded by the following grants: Howard Hughes Young Investigator Award (JDK), NIH R01 HD044745 (JDK), Illinois Excellence in Academic Medicine (Children's Memorial Institute for Education and Research), NIH R01 NS032993 (GF) and NIH R01 NS39007 (GF).

Keywords

  • Differentiation
  • Dlx genes
  • Forebrain development
  • Homeodomain
  • Immature neurons
  • Non-coding RNA
  • Nuclear RNA
  • Sonic hedgehog
  • Telencephalon
  • Ventral forebrain

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Developmental Biology

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