Abstract
We previously reported that sonic hedgehog (Shh) induces the differentiation of rat ventral forebrain neurons expressing a novel marker, EVF-1 [Development 125 (1998) 5079]. In this report, we show that EVF-1 is a novel, developmentally regulated, non-coding RNA, with no homology to other known non-coding RNA sequences. Sequence analysis, in vitro translation, and comparison of the rat and mouse EVF-1 sequences suggest that EVF-1 contains no protein coding regions. Chromosomal location indicates that EVF-1 maps adjacent to the Dlx6 gene on mouse chromosome 6. RNA in situ hybridization of the embryonic rat forebrain shows that EVF-1 is expressed by immature neurons in the subventricular zone and its expression decreases during forebrain development. Whole mount in situ hybridization shows that EVF-1 is expressed at high levels in the branchial arches, ventral forebrain, olfactory bulb, and limbs. EVF-1 expression is linked to Shh and the Dlx family of proteins, genes with a demonstrated importance to ventral forebrain and craniofacial development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 407-412 |
| Number of pages | 6 |
| Journal | Gene Expression Patterns |
| Volume | 4 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jul 2004 |
Funding
This work was funded by the following grants: Howard Hughes Young Investigator Award (JDK), NIH R01 HD044745 (JDK), Illinois Excellence in Academic Medicine (Children's Memorial Institute for Education and Research), NIH R01 NS032993 (GF) and NIH R01 NS39007 (GF).
Keywords
- Differentiation
- Dlx genes
- Forebrain development
- Homeodomain
- Immature neurons
- Non-coding RNA
- Nuclear RNA
- Sonic hedgehog
- Telencephalon
- Ventral forebrain
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Developmental Biology