Developmental regulation of Zbu1, a DNA-binding member of the SWI2/SNF2 family

Xiaohua Gong, Sunjay Kaushal, Elena Ceccarelli, Natalia Bogdanova, Craig Neville, Tom Nguyen, Hilary Clark, Ziad A. Khatib, Marcus Valentine, A. Thomas Look, Nadia Rosenthal*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

The SWI2/SNF2 gene family has been implicated in a wide variety of processes, involving regulation of DNA structure and chromatin configuration, mitotic chromosome segregation, and DNA repair. Here we report the characterization of the Zbu1 gene, also known as HIP116, located on human chromosome band 3q25, which encodes a DNA-binding member of this superfamily. Zbu1 was isolated in this study by its affinity for a site in the myosin light chain 1/3 enhancer. The protein has single-stranded DNA-dependent ATPase activity, includes seven helicase motifs, and a RING finger motif that is shared exclusively by the R-AD5, spRAD8, and RAD16 family members. During mouse embryogenesis, Zbu1 transcripts are detected relatively late in fetal development and increase in neonatal stages, whereas the protein accumulates asynchronously in heart, skeletal muscle, and brain. In adult human tissues, alternatively spliced Zbu1 transcripts are ubiquitous with highest expression in these tissues. Gene expression is also dramatically induced in human tumor lines and in Li-Fraumeni fibroblast cultures, suggesting that it is aberrantly regulated in malignant cells. The developmental profile of Zbu1 gene expression and the association of the protein with a tissue-specific transcriptional regulatory element distinguish it from other members of the SWI2/SNE2 family and suggest novel roles for the Zbu1 gene product.

Original languageEnglish (US)
Pages (from-to)166-182
Number of pages17
JournalDevelopmental Biology
Volume183
Issue number2
DOIs
StatePublished - Mar 15 1997
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Developmental regulation of Zbu1, a DNA-binding member of the SWI2/SNF2 family'. Together they form a unique fingerprint.

Cite this