Developmentally regulated expression of calponin isoforms and the effect of h2-calponin on cell proliferation

M. Moazzem Hossain, Daw Yang Hwang, Qi Quan Huang, Yasuharu Sasaki, Jian Ping Jin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

h2-Calponin is found in both smooth muscle and nonmuscle cells, and its function remains to be established. Western blots with specific monoclonal antibodies detected significant expression of h2-calponin in the growing embryonic stomach and urinary bladder and the early pregnant uterus. Although the expression of h1-calponin is upregulated in the stomach and bladder during postnatal development, the expression of h2-calponin is decreased to low levels in quiescent smooth muscle cells. To investigate a hypothesis that h2-calponin regulates the function of the actin cytoskeleton during cytokinesis, a smooth muscle-originated cell line (SM3) lacking calponin was transfected to express either sense or antisense h2-calponin cDNA and the effects on the rates of cell proliferation were examined. Both stable and transient sense cDNA-transfected cells had a significantly decreased proliferation rate compared with the antisense cDNA-transfected or nontransfected cells. Immunofluorescence microscopy showed that the force-expressed h2-calponin was associated with actin-tropomyosin microfilaments. The number of binuclear cells was significantly greater in the sense cDNA-transfected culture, in which h2-calponin was concentrated in a nuclear ring structure formed by actin filaments. The results suggest that h2-calponin may regulate cytokinesis by inhibiting the activity of the actin cytoskeleton.

Original languageEnglish (US)
Pages (from-to)C156-C167
JournalAmerican Journal of Physiology - Cell Physiology
Volume284
Issue number1 53-1
DOIs
StatePublished - Jan 1 2003

Keywords

  • Actin cytoskeleton
  • Cytokinesis
  • Monoclonal antibody
  • Smooth muscle development
  • Transfective expression
  • Tropomyosin

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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