Dexamethasone upregulates the Na-K-ATPase in rat alveolar epithelial cells

Nora Barquin, David E. Ciccolella, Karen M. Ridge, J. Iasha Sznajder*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Previous studies in kidney, heart, and liver cells have demonstrated that dexamethasone regulates the expression of Na-K-ATPase. In the lungs, Na- K-ATPase has been reported in alveolar epithelial type II (ATII) cells and is thought to participate in active Na+ transport and lung edema clearance. The aim of this study was to determine whether Na-K-ATPase would be regulated by dexamethasone in cultured rat ATII cells. Regulation of the Na-K-ATPase by dexamethasone could lead to a greater understanding of its role in active Na+ transport and lung edema clearance. Rat ATII cells were isolated, plated for 24 h, and exposed to 10-7 and 10-8 M dexamethasone. These cells were harvested at 0, 3, 6, 12, and 24 h after dexamethasone exposure for determination of steady-state Na-K-ATPase mRNA transcript levels, protein expression, and function. The steady-state Na-K-ATPase β1-mRNA transcript levels increased in ATII cells 6, 12, and 24 h after dexamethasone exposure (P < 0.05). However, the steady-state α1-mRNA transcript levels were unchanged. The protein expression for the α1- and β1-subunits increased in ATII cells exposed to dexamethasone compared with controls in association with a temporal increase in Na-K-ATPase function after dexamethasone exposure. These results suggest that dexamethasone regulates Na-K-ATPase in ATII cells possibly by transcriptional, translational, and posttranslational mechanisms.

Original languageEnglish (US)
Pages (from-to)L825-L830
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume273
Issue number4 17-4
DOIs
StatePublished - 1997

Keywords

  • Alveolar type II cells
  • Regulation of sodium-potassium- adenosinetriphosphatase in lungs
  • Sodium-potassium- adenosinetriphosphatase

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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