Diacylglycerol formation and DNA synthesis in endothelin-stimulated rat C6 glioma cells: the possible role of phosphatidylcholine breakdown

Wei Zhang*, Noboru Sakai, Tao Fu, Yukio Okano, Hiroshi Hirayama, Katsunobu Takenaka, Hiromu Yamada, Yoshinori Nozawa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Stimulation of C6 cells with endothelin-1 (ET) caused a biphasic sn-1,2-diacylglycerol (1,2-DG) generation, which occurred not only via phosphatidylinositol 4,5-P2 (PIP2) hydrolysis by specific phospholipase C action, but also through the breakdown of phosphatidylcholine (PC) induced by either PC phospholipase C or D. ET also stimulated DNA synthesis in serum-starved C6 cells. However, in the presence of 1-(5-isoquinolynylsulfonyl)-2-methylpiperazine (H-7), a protein kinase C (PKC) inhibitor, the [3H]thymidine incorporation was markedly inhibited, which was concurrent with the 1,2-DG formation: the second peak was reduced. These findings suggest that brain glial cells might be an important target for ET. In addition to other possible roles. ET may also mediate mitogenesis in the brain, presumably through a PKC-dependent activation of phospholipase C and/or D hydrolyzing PC.

Original languageEnglish (US)
Pages (from-to)164-166
Number of pages3
JournalNeuroscience Letters
Volume123
Issue number2
DOIs
StatePublished - Feb 25 1991

Keywords

  • DNA synthesis
  • Diacylglycerol
  • Endothelin-1
  • Glioma cell
  • Phosphatidylcholine
  • Protein kinase C

ASJC Scopus subject areas

  • Neuroscience(all)

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