Diagnostic Performance of Ion 16S Metagenomics Kit and Ion Reporter Metagenomics Workflow for Bacterial Pathogen Detection in Culture-Negative Clinical Specimens From Sterile Sources

Michael Malczynski, Alex Zhu, Teresa R Zembower, Chao Qi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

PCR-based deep sequencing of 16S rRNA gene allows for detection of a wide array of bacterial pathogens in culture-negative specimens. Ion 16S metagenomics kit and Ion Reporter metagenomics workflow (Ion 16S mNGS) provides an end-to-end solution with integrated workflow. Ninety-eight clinical samples with the diagnosis generated with 16S rRNA gene PCR/chain termination (Sanger) sequencing (16S CS) was used to assess the performance of Ion 16S mNGS. Compared to species level detection of 16S CS, the Ion 16S mNGS had 88% sensitivity and 76% specificity. When accounting for genus level of detection, the Ion 16S mNGS had 100% sensitivity. Notably, Ion 16S mNGS generated diagnosis in 13% of 16S CS and culture-negative samples. In addition, Ion 16S mNGS had the advantage of detecting more than 1 pathogen in 16S CS positive samples. We showed that the workflow had high reproducibility.

Original languageEnglish (US)
Article number115451
JournalDiagnostic Microbiology and Infectious Disease
Volume101
Issue number2
DOIs
StatePublished - Oct 2021

Keywords

  • 16S CS, 16S rRNA gene PCR/chain termination (Sanger) sequencing
  • Ion Reporter metagenomics workflow, Bacterial pathogen detection, Culture-negative clinical specimens. Abbreviations: Ion 16S mNGS, 16S metagenomics kit and Ion Reporter metagenomics workflow

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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