Abstract
We developed a post-embedding ultrastructural enzyme affinity-gold technique to label histamine. Diamine oxidase (DAO)-gold complex was prepared and tested for specificity with routinely processed, Epon-embedded, histamine-rich human mast-cell granules and histamine-agar test blocks, both of which were labeled. Specificity controls included removal of staining by DAO digestion of Epon sections containing mast cells or by filtering DAO- gold over histamine-agarose beads, and a wide variety of controls (for these specificity controls) that did not abrogate mast-cell granule or histamine- agar block labeling with DAO-gold. Internal negative controls in each sample included the failure of DAO-gold to stain Type II alveolar pneumocyte lamellar bodies or altered, swollen mast-cell granules from which histamine has been released. The new post-embedding enzyme affinity-gold technique is effective in optimally prepared ultrastructural samples that allow clear identification of key ultrastructural morphology.
Original language | English (US) |
---|---|
Pages (from-to) | 787-800 |
Number of pages | 14 |
Journal | Journal of Histochemistry and Cytochemistry |
Volume | 41 |
Issue number | 6 |
DOIs | |
State | Published - 1993 |
Keywords
- Diamine oxidase
- Histaminase
- Histamine
- Mast cells
- Secretory granules
- Type II alveolar pneumocytes
- Ultrastructural enzyme affinity-gold method
ASJC Scopus subject areas
- Anatomy
- Histology