Dicer1 is required for differentiation of the mouse male germline

Danielle M. Maatouk, Kate L. Loveland, Michael T. McManus, Karen Moore, Brian D. Harfe

Research output: Contribution to journalArticlepeer-review

191 Scopus citations

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that posttranscriptionally regulate gene expression. Hundreds of miRNAs are expressed in mammals; however, their functions are just starting to be uncovered. MicroRNAs are processed from a long hairpin mRNA transcript, down to a ∼23-nucleotide duplex. The enzyme Dicer1 is required for miRNA processing, and mouse knockouts of Dicer1 are embryonic lethal before 7.5 days postcoitus. To examine the function of miRNAs specifically in the germline, we used a mouse model that expresses Cre recombinase from the TNAP locus and a floxed Dicer1 conditional allele. Removal of Dicer1 from germ cells resulted in male infertility. Germ cells were present in adult testes, but few tubules contained elongating spermatids. Germ cells that did differentiate to elongating spermatids exhibited abnormal morphology and motility. Rarely, sperm lacking Dicer1 could fertilize wild-type eggs to generate viable offspring. These results show that Dicer1 and miRNAs are essential for proper differentiation of the male germline.

Original languageEnglish (US)
Pages (from-to)696-703
Number of pages8
JournalBiology of reproduction
Volume79
Issue number4
DOIs
StatePublished - Oct 2008

Keywords

  • Dicer
  • MicroRNA
  • Sperm maturation
  • Spermatogenesis
  • Testis

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

Fingerprint

Dive into the research topics of 'Dicer1 is required for differentiation of the mouse male germline'. Together they form a unique fingerprint.

Cite this