DICER1 loss and Alu RNA induce age-related macular degeneration via the NLRP3 inflammasome and MyD88

Valeria Tarallo, Yoshio Hirano, Bradley D. Gelfand, Sami Dridi, Nagaraj Kerur, Younghee Kim, Won Gil Cho, Hiroki Kaneko, Benjamin J. Fowler, Sasha Bogdanovich, Romulo J.C. Albuquerque, William W. Hauswirth, Vince A. Chiodo, Jennifer F. Kugel, James A. Goodrich, Steven L. Ponicsan, Gautam Chaudhuri, Michael P. Murphy, Joshua L. Dunaief, Balamurali K. AmbatiYuichiro Ogura, Jae Wook Yoo, Dong Ki Lee, Patrick Provost, David R. Hinton, Gabriel Núñez, Judit Z. Baffi, Mark E. Kleinman, Jayakrishna Ambati*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

365 Scopus citations

Abstract

Alu RNA accumulation due to DICER1 deficiency in the retinal pigmented epithelium (RPE) is implicated in geographic atrophy (GA), an advanced form of age-related macular degeneration that causes blindness in millions of individuals. The mechanism of Alu RNA-induced cytotoxicity is unknown. Here we show that DICER1 deficit or Alu RNA exposure activates the NLRP3 inflammasome and triggers TLR-independent MyD88 signaling via IL18 in the RPE. Genetic or pharmacological inhibition of inflammasome components (NLRP3, Pycard, Caspase-1), MyD88, or IL18 prevents RPE degeneration induced by DICER1 loss or Alu RNA exposure. These findings, coupled with our observation that human GA RPE contains elevated amounts of NLRP3, PYCARD, and IL18 and evidence of increased Caspase-1 and MyD88 activation, provide a rationale for targeting this pathway in GA. Our findings also reveal a function of the inflammasome outside the immune system and an immunomodulatory action of mobile elements.

Original languageEnglish (US)
Pages (from-to)847-859
Number of pages13
JournalCell
Volume149
Issue number4
DOIs
StatePublished - May 11 2012

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'DICER1 loss and Alu RNA induce age-related macular degeneration via the NLRP3 inflammasome and MyD88'. Together they form a unique fingerprint.

Cite this