Dielectrophoretic concentration of low-abundance nanoparticles using a nanostructured tip

Woon Hong Yeo*, Adrian M. Kopacz, Jong Hoon Kim, Xinqi Chen, Jinsong Wu, Dayong Gao, Kyong Hoon Lee, Wing Kam Liu, Jae Hyun Chung

*Corresponding author for this work

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Electric field-induced concentration has the potential for application in highly sensitive detection of nanoparticles (NPs) for disease diagnosis and drug discovery. Conventional two-dimensional planar electrodes, however, have shown limited sensitivity in NP concentration. In this paper, the dielectrophoretic (DEP) concentration of low-abundance NPs is studied using a nanostructured tip where a high electric field of 3×10 7Vm 1 is generated. In experimental studies, individual 2, 10, and 100nm Au NPs are concentrated to a nanotip using DEP concentration and are detected by scanning transmission and scanning electron microscopes. The DEP force on Au NPs near the end of a nanotip is computed according to the distance, and then compared with Brownian motion-induced force. The computational study shows qualitative agreement with the experimental results. When the experimental conditions for DEP concentration are optimized for 8nm-long oligonucleotides, the sensitivity of a nanotip is 10aM (10 attomolar; nine copies in a 1.5μl sample volume). This DEP concentrator using a nanotip can be used for molecular detection without amplification.

Original languageEnglish (US)
Article number485707
JournalNanotechnology
Volume23
Issue number48
DOIs
StatePublished - Dec 7 2012

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)

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