Dietary apigenin reduces LPS-induced expression of miR-155 restoring immune balance during inflammation

Daniel Arango, Mayra Diosa-Toro, Laura S. Rojas-Hernandez, Jessica L. Cooperstone, Steven J. Schwartz, Xiaokui Mo, Jinmai Jiang, Thomas D. Schmittgen, Andrea I. Doseff*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

63 Scopus citations


Scope: High incidence of inflammatory diseases afflicts the increasing aging-population infringing a great health burden. Dietary flavonoids, including the flavone apigenin, are emerging as important anti-inflammatory nutraceuticals due to their health benefits, lack of adverse effects and reduced costs. MicroRNAs (miRs) play a central role in inflammation by regulating gene expression, yet how dietary ingredients affect miRs is poorly understood. The aim of this study was to identify miRs involved in the anti-inflammatory activity of apigenin and apigenin-rich diets and determine their immune regulatory mechanisms in macrophages and in vivo. Methods and results: A high-throughput quantitative reverse transcriptase PCR screen of 312 miRs in macrophages revealed that apigenin reduced LPS-induced miR-155 expression. Analyses of miR-155 precursor and primary transcript indicated that apigenin regulated miR-155 transcriptionally. Apigenin-reduced expression of miR-155 led to the increase of anti-inflammatory regulators forkhead box O3a and smooth-muscle-actin and MAD-related protein 2 in LPS-treated macrophages. In vivo, apigenin or a celery-based apigenin-rich diet reduced LPS-induced expression of miR-155 and decreased tumor necrosis factor α in lungs from LPS-treated mice. Conclusion: These results demonstrate that apigenin and apigenin-rich diets exert effective anti-inflammatory activity in vivo by reducing LPS-induced expression of miR-155, thereby restoring immune balance.

Original languageEnglish (US)
Pages (from-to)763-772
Number of pages10
JournalMolecular Nutrition and Food Research
Issue number4
StatePublished - Apr 1 2015
Externally publishedYes


  • Flavonoids
  • Macrophages
  • MicroRNAs
  • NF-κB
  • Sepsis

ASJC Scopus subject areas

  • Biotechnology
  • Food Science


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