Differences in SpeB protease activity among group a streptococci associated with superficial, invasive, and autoimmune disease

Anhphan T. Ly, John P. Noto, Odaelys L. Walwyn, Robert R. Tanz, Stanford T. Shulman, William Kabat, Debra E. Bessen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The secreted cysteine proteinase SpeB is an important virulence factor of group A streptococci (GAS), whereby SpeB activity varies widely among strains. To establish the degree to which SpeB activity correlates with disease, GAS organisms were recovered from patients with pharyngitis, impetigo, invasive disease or acute rheumatic fever (ARF), and selected for analysis using rigorous sampling criteria; >300 GAS isolates were tested for SpeB activity by casein digestion assays, and each GAS isolate was scored as a SpeB-producer or non-producer. Highly significant statistical differences (p < 0.01) in SpeB production are observed between GAS recovered from patients with ARF (41.5% SpeB-non-producers) compared to pharyngitis (20.5%), invasive disease (16.7%), and impetigo (5.5%). SpeB activity differences between pharyngitis and impetigo isolates are also significant, whereas pharyngitis versus invasive isolates show no significant difference. The disproportionately greater number of SpeB-non-producers among ARF-associated isolates may indicate an altered transcriptional program for many rheumatogenic strains and/or a protective role for SpeB in GAS-triggered autoimmunity.

Original languageEnglish (US)
Article numbere0177784
JournalPloS one
Volume12
Issue number5
DOIs
StatePublished - May 1 2017

Fingerprint

autoimmune diseases
Streptococcus
Autoimmune Diseases
Peptide Hydrolases
proteinases
Pharyngitis
Impetigo
Rheumatic Fever
fever
Cysteine Proteases
Virulence Factors
Caseins
Assays
Sampling
autoimmunity
cysteine proteinases
Autoimmunity
Digestion
casein
virulence

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Ly, Anhphan T. ; Noto, John P. ; Walwyn, Odaelys L. ; Tanz, Robert R. ; Shulman, Stanford T. ; Kabat, William ; Bessen, Debra E. / Differences in SpeB protease activity among group a streptococci associated with superficial, invasive, and autoimmune disease. In: PloS one. 2017 ; Vol. 12, No. 5.
@article{72e4a49214fd423bbfb5257b89bbae83,
title = "Differences in SpeB protease activity among group a streptococci associated with superficial, invasive, and autoimmune disease",
abstract = "The secreted cysteine proteinase SpeB is an important virulence factor of group A streptococci (GAS), whereby SpeB activity varies widely among strains. To establish the degree to which SpeB activity correlates with disease, GAS organisms were recovered from patients with pharyngitis, impetigo, invasive disease or acute rheumatic fever (ARF), and selected for analysis using rigorous sampling criteria; >300 GAS isolates were tested for SpeB activity by casein digestion assays, and each GAS isolate was scored as a SpeB-producer or non-producer. Highly significant statistical differences (p < 0.01) in SpeB production are observed between GAS recovered from patients with ARF (41.5{\%} SpeB-non-producers) compared to pharyngitis (20.5{\%}), invasive disease (16.7{\%}), and impetigo (5.5{\%}). SpeB activity differences between pharyngitis and impetigo isolates are also significant, whereas pharyngitis versus invasive isolates show no significant difference. The disproportionately greater number of SpeB-non-producers among ARF-associated isolates may indicate an altered transcriptional program for many rheumatogenic strains and/or a protective role for SpeB in GAS-triggered autoimmunity.",
author = "Ly, {Anhphan T.} and Noto, {John P.} and Walwyn, {Odaelys L.} and Tanz, {Robert R.} and Shulman, {Stanford T.} and William Kabat and Bessen, {Debra E.}",
year = "2017",
month = "5",
day = "1",
doi = "10.1371/journal.pone.0177784",
language = "English (US)",
volume = "12",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",

}

Differences in SpeB protease activity among group a streptococci associated with superficial, invasive, and autoimmune disease. / Ly, Anhphan T.; Noto, John P.; Walwyn, Odaelys L.; Tanz, Robert R.; Shulman, Stanford T.; Kabat, William; Bessen, Debra E.

In: PloS one, Vol. 12, No. 5, e0177784, 01.05.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Differences in SpeB protease activity among group a streptococci associated with superficial, invasive, and autoimmune disease

AU - Ly, Anhphan T.

AU - Noto, John P.

AU - Walwyn, Odaelys L.

AU - Tanz, Robert R.

AU - Shulman, Stanford T.

AU - Kabat, William

AU - Bessen, Debra E.

PY - 2017/5/1

Y1 - 2017/5/1

N2 - The secreted cysteine proteinase SpeB is an important virulence factor of group A streptococci (GAS), whereby SpeB activity varies widely among strains. To establish the degree to which SpeB activity correlates with disease, GAS organisms were recovered from patients with pharyngitis, impetigo, invasive disease or acute rheumatic fever (ARF), and selected for analysis using rigorous sampling criteria; >300 GAS isolates were tested for SpeB activity by casein digestion assays, and each GAS isolate was scored as a SpeB-producer or non-producer. Highly significant statistical differences (p < 0.01) in SpeB production are observed between GAS recovered from patients with ARF (41.5% SpeB-non-producers) compared to pharyngitis (20.5%), invasive disease (16.7%), and impetigo (5.5%). SpeB activity differences between pharyngitis and impetigo isolates are also significant, whereas pharyngitis versus invasive isolates show no significant difference. The disproportionately greater number of SpeB-non-producers among ARF-associated isolates may indicate an altered transcriptional program for many rheumatogenic strains and/or a protective role for SpeB in GAS-triggered autoimmunity.

AB - The secreted cysteine proteinase SpeB is an important virulence factor of group A streptococci (GAS), whereby SpeB activity varies widely among strains. To establish the degree to which SpeB activity correlates with disease, GAS organisms were recovered from patients with pharyngitis, impetigo, invasive disease or acute rheumatic fever (ARF), and selected for analysis using rigorous sampling criteria; >300 GAS isolates were tested for SpeB activity by casein digestion assays, and each GAS isolate was scored as a SpeB-producer or non-producer. Highly significant statistical differences (p < 0.01) in SpeB production are observed between GAS recovered from patients with ARF (41.5% SpeB-non-producers) compared to pharyngitis (20.5%), invasive disease (16.7%), and impetigo (5.5%). SpeB activity differences between pharyngitis and impetigo isolates are also significant, whereas pharyngitis versus invasive isolates show no significant difference. The disproportionately greater number of SpeB-non-producers among ARF-associated isolates may indicate an altered transcriptional program for many rheumatogenic strains and/or a protective role for SpeB in GAS-triggered autoimmunity.

UR - http://www.scopus.com/inward/record.url?scp=85019875531&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019875531&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0177784

DO - 10.1371/journal.pone.0177784

M3 - Article

C2 - 28545045

AN - SCOPUS:85019875531

VL - 12

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 5

M1 - e0177784

ER -