Differences in the effects of membrane depolarization on levels of preprosomatostatin mRNA and tyrosine hydroxylase mRNA in rat sympathetic neurons in vivo and in culture

Katharyn Spiegel, Norbert E. Kremer, John A. Kessler*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Regulation of preprosomatostatin mRNA and tyrosine hydroxylase mRNA were examined in sympathetic neurons of the rat superior cervical ganglion (SCG). Surgical denervation of the adult SCG increased ganglion levels of preprosomatostatin (SS) mRNA more than 11-fold, and levels of the mRNA remained elevated 14 days after surgery. By contrast, denervation decreased levels of tyrosine hydroxylase (TH) mRNA. Potassium- or veratridine-induced membrane depolarization of cultured neonatal sympathetic neurons decreased levels of SS mRNA but elevated levels of TH mRNA. Sodium channel blockade with tetrodotoxin prevented the effects of veratridine on SS and TH mRNAs. In toto these observations suggest that transsynaptic nerve impulse activity and sympathetic neurons membrane depolarization decrease SS synthesis but increase TH synthesis at the mRNA level. Thus nerve impulse activity may alter the relative levels of different transmitters co-expressed in the same neuronal population by inhibiting levels of some species of mRNA while simultaneously stimulating levels of others.

Original languageEnglish (US)
Pages (from-to)23-29
Number of pages7
JournalMolecular Brain Research
Volume5
Issue number1
DOIs
StatePublished - Jan 1989

Keywords

  • Membrane depolarization
  • Nerve impulse activity
  • Phenotypic expression
  • Somatostatin
  • Sympathetic neuron
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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