Different aa-tRNAs Are Selected Uniformly on the Ribosome

Sarah Ledoux; Olke C. Uhlenbeck

doi:10.1016/j.molcel.2008.04.026

Different aa-tRNAs Are Selected Uniformly on the Ribosome

Sarah Ledoux, Olke C. Uhlenbeck^*

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

Ten E. coli aminoacyl-tRNAs (aa-tRNAs) were assessed for their ability to decode cognate codons on E. coli ribosomes by using three assays that evaluate the key steps in the decoding pathway. Despite a wide variety of structural features, each aa-tRNA exhibited similar kinetic and thermodynamic properties in each assay. This surprising kinetic and thermodynamic uniformity is likely to reflect the importance of ribosome conformational changes in defining the rates and affinities of the decoding process as well as the evolutionary "tuning" of each aa-tRNA sequence to modify their individual interactions with the ribosome at each step.

Original language	English (US)
Pages (from-to)	114-123
Number of pages	10
Journal	Molecular cell
Volume	31
Issue number	1
DOIs	https://doi.org/10.1016/j.molcel.2008.04.026
State	Published - Jul 11 2008

Keywords

RNA

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/j.molcel.2008.04.026

Cite this

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abstract = "Ten E. coli aminoacyl-tRNAs (aa-tRNAs) were assessed for their ability to decode cognate codons on E. coli ribosomes by using three assays that evaluate the key steps in the decoding pathway. Despite a wide variety of structural features, each aa-tRNA exhibited similar kinetic and thermodynamic properties in each assay. This surprising kinetic and thermodynamic uniformity is likely to reflect the importance of ribosome conformational changes in defining the rates and affinities of the decoding process as well as the evolutionary {"}tuning{"} of each aa-tRNA sequence to modify their individual interactions with the ribosome at each step.",

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Different aa-tRNAs Are Selected Uniformly on the Ribosome

Abstract

Keywords

ASJC Scopus subject areas

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