Differential activation of individual subunits in heteromeric kainate receptors

Geoffrey T. Swanson, Tim Green, Ryuichi Sakai, Anis Contractor, Wesley Che, Hisao Kamiya, Stephen F. Heinemann

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

Neuronal kainate receptors are assembled from subunits with dissimilar specificities for agonists and antagonists. The composite biophysical behavior of heteromeric kainate receptors is determined by intersubunit interactions whose nature is unclear. Here we use dysiherbaine, a selective kainate receptor agonist, to show that GluR5 subunits assembled in heteromeric GluR5/KA-2 kainate receptor complexes can gate current without concomitant activation of their partner KA-2 subunits. A long-lasting interaction between dysiherbaine and GluR5 subunits elicits a tonic current from GluR5/KA-2 receptors; subsequent cooperative gating of KA-2 subunits can be elicited by both agonists, such as glutamate, and some classically defined antagonists, such as CNQX. This study demonstrates that each type of subunit within a heteromeric kainate receptor contributes a distinct conductance upon activation by agonist binding, and therefore provides insight into the biophysical function of ionotropic glutamate receptors.

Original languageEnglish (US)
Pages (from-to)589-598
Number of pages10
JournalNeuron
Volume34
Issue number4
DOIs
StatePublished - May 16 2002

ASJC Scopus subject areas

  • Neuroscience(all)

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