Differential antero-posterior expression of two proteins encoded by a homeobox gene in Xenopus and mouse embryos.

G. Oliver*, C. V. Wright, J. Hardwicke, E. M. De Robertis

*Corresponding author for this work

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

The X.laevis XlHbox 1 gene uses two functional promoters to produce a short and a long protein, both containing the same homeodomain. In this report we use specific antibodies to localize both proteins in frog embryos. The antibodies also recognize the homologous proteins in mouse embryos. In both mammalian and amphibian embryos, expression of the long protein starts more posteriorly than that of the short protein. This difference in spatial expression applies to the nervous system, the segmented mesoderm and the internal organs. This suggests that each promoter from this gene has precisely restricted regions of expression along the anterior-posterior axis of the embryo. Because the long and short proteins share a common DNA-binding specificity but differ by an 82 amino acid domain, their differential distribution may have distinct developmental consequences.

Original languageEnglish (US)
Pages (from-to)3199-3209
Number of pages11
JournalThe EMBO journal
Volume7
Issue number10
DOIs
StatePublished - Oct 1988

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Differential antero-posterior expression of two proteins encoded by a homeobox gene in Xenopus and mouse embryos.'. Together they form a unique fingerprint.

  • Cite this