Differential chemotactic potential of mouse platelet basic protein for thymocyte subsets

W. X. Fu, S. Y. Gong, X. P. Qian, Y. Li, M. L. Zhu, X. Y. Dong, Y. Li, W. F. Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Mouse platelet basic protein (CXCL7/mPBP) was cloned from thymic stromal cells and further identification indicated that it was expressed in thymic monocytes/macrophages (Mo/Mφs). Recombinant mPBP was chemoattractive for target cells of polymorphonuclear leucocytes, peritoneal Mo/Mφs and splenic lymphocytes with distinct potencies. CXCR2 was identified to be a cognate receptor for mPBP. Mouse thymocyte subsets of CD4-CD8- double-negative (DN), CD4+CD8+ double-positive (DP), CD4+CD8 single-positive (CD4SP) and CD4-CD8+ single-positive (CD8SP) expressed cell surface CXCR2 with different positive percentages and expression levels. mPBP was chemoattractive for thymocyte subsets with the potency order DN>DP> CD8SP>CD4SP, consistent with the levels of CXCR2 expressed on the respective cells. Thus, mPBP in thymus is functionally redundant with chemokine CXCL12/ SDF-1. Moreover, our finding that thymic Mo/Mφs can produce mPBP implies that they may have other functions apart from acting as scavengers in thymus.

Original languageEnglish (US)
Pages (from-to)1935-1945
Number of pages11
JournalCellular and Molecular Life Sciences
Volume61
Issue number15
DOIs
StatePublished - Aug 2004

Keywords

  • Chemokine
  • Chemotaxis
  • Monocyte/macrophage
  • Stromal cell
  • Thymus

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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