Differential control of neonatal tolerance by antigen dose versus extended exposure and adjuvant

Booki Min*, Kevin L. Legge, Jacque C. Caprio, Lequn Li, Randal Gregg, Habib Zaghouani

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Ig-PLP1, an immunoglobulin (Ig) chimera carrying the encephalitogenic proteolipid protein (PLP) sequence 139-151 (PLP1), induces neonatal tolerance in mice and confers resistance to experimental allergic encephalomyelitis (EAE) without the need for incomplete Freund's adjuvant (IFA). The mechanism underlying such tolerance involves organ-specific T cell regulation characterized by lymph node deviation and an unusual IFNγ-dependent splenic anergy. This form of T cell modulation may prove useful for prevention of autoimmunity. However, since the neonatal period is susceptible to regulation, further investigations are necessary to define parameters required to establish regimens suitable for optimal protection against disease. Therefore, studies were carried out to investigate the effect that IFA, the dose of Ig-PLP1, and the number of Ig-PLP1 injections might have on Ig-PLP1-mediated neonatal tolerance and protection against disease. Herein it is reported that as little as 1 μg of Ig-PLP1 supported IFNγ-dependent splenic anergy but lymph node deviation and protection against disease strengthened as the dose of tolerogen increased. However, when a two- injection regimen was applied, resistance to disease was observed but the mechanism manifested proliferative and cytokine unresponsiveness in both lymphoid organs. Furthermore, the use of IFA along with Ig-PLP1 yielded a suppressive mechanism similar to that of the two-injection regimen. Therefore, the dose of Ig-PLP1 displays a quantitative influence, while the number of injections of Ig-PLP1 and the presence of IFA rather drive qualitative influences on such tolerance. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)45-55
Number of pages11
JournalCellular Immunology
Issue number1
StatePublished - Feb 25 2000
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'Differential control of neonatal tolerance by antigen dose versus extended exposure and adjuvant'. Together they form a unique fingerprint.

Cite this