Differential effect of Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1) on leukocyte infiltration during contact hypersensitivity responses

Merideth Early, William G. Schroeder, Ranajana Unnithan, John M. Gilchrist, William A. Muller, Alan Schenkel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Background. 2'-4' Dinitrofluorobenzene (DNFB) induced contact hypersensitivity is an established model of contact sensitivity and leukocyte migration. Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1) deficient mice were used to examine the role of PECAM-1 in the migration capacity of several different leukocyte populations after primary and secondary application. Results. γδ T lymphocytes, granulocytes, and Natural Killer cells were most affected by PECAM-1 deficiency at the primary site of application. γδ T lymphocytes, granulocytes, DX5+ Natural Killer cells, and, interestingly, effector CD4+ T lymphocytes were most affected by the loss of PECAM-1 at the secondary site of application. Conclusions. PECAM-1 is used by many leukocyte populations for migration, but there are clearly differential effects on the usage by each subset. Further, the overall kinetics of each population varied between primary and secondary application, with large relative increases in γδ T lymphocytes during the secondary response.

Original languageEnglish (US)
Article numbere3555
JournalPeerJ
Volume2017
Issue number7
DOIs
StatePublished - 2017

Keywords

  • CD4+ T lymphocytes
  • Contact Hypersensitivity
  • Emigration
  • Granulocytes
  • Leukocyte
  • Monocytes
  • Natural Killer Lymphocytes
  • Platelet Endothelial Cell Adhesion Molecule-1
  • γδT lymphocytes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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