Abstract
Aim: Airway epithelial cells play important roles in innate immunity and inflammatory responses in the airways. We investigated the effect of the potent inhaled glucocorticoid FP on expressions of both innate immune effectors and inflammatory mediators in BEAS-2 B airway epithelial cells after stimulation with Toll-like receptor 3 ligand double stranded RNA (dsRNA). Methods: BEAS-2B airway epithelial cells were treated with FP (10-7 mol · L-1) for 2 h before stimulation with dsRNA (25 mg · L -1). RNA was isolated and the cell supernant was collected. mRNA for the inflammatory cytokines IL-8, GM-CSF, IL-1β and the innate immunity effectors serum amyloid A (SAA), complement B factor, secretory leukocyte protease inhibitor (SLPI) were quantified by real-time RT-PCR. The protein level of IL-8, GM-CSF and SAA in the supernant was analyzed by ELISA. Results: Stimulation of BEAS-2B cells with dsRNA increased the mRNA levels of IL-8, GM-CSF, IL-1β, SAA, B factor and SLPI. Treatment with FP did not inhibit induction of mRNA for SAA, B factor and SLPI. FP did inhibit expressions of mRNA for IL-8, GM-CSF, IL-1β. ELISA of IL-8, GM-CSF and SAA confirmed the mRNA expression results. Conclusion: Our results suggest that FP may spare innate responses in airway epithelial cells while it inhibit expression of inflammatory mediators.
Original language | English (US) |
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Pages (from-to) | 67-70 |
Number of pages | 4 |
Journal | Chinese Pharmacological Bulletin |
Volume | 22 |
Issue number | 1 |
State | Published - Jan 2006 |
Keywords
- Airway epithelial cells
- Fluticasone propionate
- Inflammatory cytokines
- Inhaled glucocorticoids
- Innate immunity
ASJC Scopus subject areas
- Pharmacology