Differential effects of fluticasone propionate (FP) on gene expression in airway epithelial cells

Quan Sha*, Robert P. Schleimer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Aim: Airway epithelial cells play important roles in innate immunity and inflammatory responses in the airways. We investigated the effect of the potent inhaled glucocorticoid FP on expressions of both innate immune effectors and inflammatory mediators in BEAS-2 B airway epithelial cells after stimulation with Toll-like receptor 3 ligand double stranded RNA (dsRNA). Methods: BEAS-2B airway epithelial cells were treated with FP (10-7 mol · L-1) for 2 h before stimulation with dsRNA (25 mg · L -1). RNA was isolated and the cell supernant was collected. mRNA for the inflammatory cytokines IL-8, GM-CSF, IL-1β and the innate immunity effectors serum amyloid A (SAA), complement B factor, secretory leukocyte protease inhibitor (SLPI) were quantified by real-time RT-PCR. The protein level of IL-8, GM-CSF and SAA in the supernant was analyzed by ELISA. Results: Stimulation of BEAS-2B cells with dsRNA increased the mRNA levels of IL-8, GM-CSF, IL-1β, SAA, B factor and SLPI. Treatment with FP did not inhibit induction of mRNA for SAA, B factor and SLPI. FP did inhibit expressions of mRNA for IL-8, GM-CSF, IL-1β. ELISA of IL-8, GM-CSF and SAA confirmed the mRNA expression results. Conclusion: Our results suggest that FP may spare innate responses in airway epithelial cells while it inhibit expression of inflammatory mediators.

Original languageEnglish (US)
Pages (from-to)67-70
Number of pages4
JournalChinese Pharmacological Bulletin
Volume22
Issue number1
StatePublished - Jan 2006

Keywords

  • Airway epithelial cells
  • Fluticasone propionate
  • Inflammatory cytokines
  • Inhaled glucocorticoids
  • Innate immunity

ASJC Scopus subject areas

  • Pharmacology

Fingerprint Dive into the research topics of 'Differential effects of fluticasone propionate (FP) on gene expression in airway epithelial cells'. Together they form a unique fingerprint.

Cite this