Differential expression of keratin 5 gene in non-tumorigenic and tumorigenic rat bladder cell lines

Li Nan, Hitoshi Kawamata, Xiaodi Tan, Shuji Kameyama, Ryoichi Oyasu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

In this study, we attempted to find a gene or genes which were differentially expressed between a non-tumorigenic rat bladder cell line and a highly tumorigenic/metastatic bladder carcinoma cell line that was derived from the former after treatment in vitro with N-methyl-N-nitrosourea. We cloned a rat keratin 5 cDNA by a differential hybridization technique and found that all of the non-tumorigenic cells (7/7) and normal bladder tissue expressed keratin 5, but most of the tumorigenic cells (8/10) did not express keratin 5. Furthermore, in a spontaneously transformed cell line, keratin 5 expression was lost during the transformation process. These results suggest that loss of keratin 5 expression is closely associated with acquisition of a tumorigenic phenotype by rat bladder non-tumorigenic cells.

Original languageEnglish (US)
Pages (from-to)87-93
Number of pages7
JournalCancer Letters
Volume75
Issue number2
DOIs
StatePublished - Dec 10 1993

Keywords

  • Bladder carcinoma
  • Cytokeratin
  • Differential expression

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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