TY - JOUR
T1 - Differential Expression of Ly6C and T-bet Distinguish Effector and Memory Th1 CD4 + Cell Properties during Viral Infection
AU - Marshall, Heather D.
AU - Chandele, Anmol
AU - Jung, Yong Woo
AU - Meng, Hailong
AU - Poholek, Amanda C.
AU - Parish, Ian A.
AU - Rutishauser, Rachel
AU - Cui, Weiguo
AU - Kleinstein, Steven H.
AU - Craft, Joe
AU - Kaech, Susan M.
N1 - Funding Information:
The authors would like to thank C. Dominguez, C. Perry, and other members of the S.M.K. and J.C. labs for helpful comments and technical expertise. We also thank M. Shlomchik for use of his fluorescent microscope and H. Hengartner for Stg transgenic mice supplied by R. Ahmed. This work was supported by NIH RO1 AI074699 (S.M.K.) and NIH AI07019 (H.D.M.).
PY - 2011/10/28
Y1 - 2011/10/28
N2 - CD4 + T cells differentiate into multiple effector types, but it is unclear how they form memory T cells during infection in vivo. Profiling virus-specific CD4 + T cells revealed that effector cells with T helper 1 (Th1) or T follicular helper (Tfh) cell characteristics differentiated into memory cells, although expression of Tfh cell markers declined over time. In contrast to virus-specific effector CD8 + T cells, increased IL-7R expression was not a reliable marker of CD4 + memory precursor cells. However, decreased Ly6C and T-bet (Tbx21) expression distinguished a subset of Th1 cells that displayed greater longevity and proliferative responses to secondary infection. Moreover, the gene expression profile of Ly6C loT-bet int Th1 effector cells was virtually identical to mature memory CD4 + T cells, indicating early maturation of memory CD4 + T cell features in this subset during acute viral infection. This study provides a framework for memory CD4 + T cell development after acute viral infection.
AB - CD4 + T cells differentiate into multiple effector types, but it is unclear how they form memory T cells during infection in vivo. Profiling virus-specific CD4 + T cells revealed that effector cells with T helper 1 (Th1) or T follicular helper (Tfh) cell characteristics differentiated into memory cells, although expression of Tfh cell markers declined over time. In contrast to virus-specific effector CD8 + T cells, increased IL-7R expression was not a reliable marker of CD4 + memory precursor cells. However, decreased Ly6C and T-bet (Tbx21) expression distinguished a subset of Th1 cells that displayed greater longevity and proliferative responses to secondary infection. Moreover, the gene expression profile of Ly6C loT-bet int Th1 effector cells was virtually identical to mature memory CD4 + T cells, indicating early maturation of memory CD4 + T cell features in this subset during acute viral infection. This study provides a framework for memory CD4 + T cell development after acute viral infection.
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U2 - 10.1016/j.immuni.2011.08.016
DO - 10.1016/j.immuni.2011.08.016
M3 - Article
C2 - 22018471
AN - SCOPUS:80755163540
SN - 1074-7613
VL - 35
SP - 633
EP - 646
JO - Immunity
JF - Immunity
IS - 4
ER -
