Differential expression of neural cell adhesion molecule (NCAM) after tenotomy in rabbit skeletal muscle

Amir A. Jamali, Pouya Afshar, Reid A. Abrams, Richard L. Lieber

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Tenotomy is a commonly encountered event in orthopaedic surgery. In 23 rabbit extensor digitorum longus (EDL) muscles, within 24 h after tenotomy, a marked drop in maximum force production occurred. This was not explainable based on architectural changes and histological examination using standard markers for muscle injury, i.e., haematoxylin and eosin morphology, developmental myosin heavy chain (MHC) immunolabeling, and quantitation of muscle fiber type percentage, area and distribution. The expression of neural cell adhesion molecule (NCAM), a glycoprotein expressed during muscle development was measured as a function of time in these muscles. NCAM expression was increased as early as one day after tenotomy with 2.2 ± 1.2% of the fibers showing positive expression. This expression level increased significantly to 15.4 ± 15.2% after 7 days and then subsided to 13.2 ± 10.6% 21 days after tenotomy. Two-way analysis of variance demonstrated a significant effect of time and a significant time x tenotomy method interaction. These results suggest that tenotomy leads to possible changes in muscle-nerve connections and/or excitation-contraction (EC) coupling.

Original languageEnglish (US)
Pages (from-to)364-369
Number of pages6
JournalJournal of Orthopaedic Research
Issue number2
StatePublished - 2002


  • Developmental myosin
  • Excitation-contraction coupling
  • Muscle injury
  • Muscle mechanics
  • Surgical tendon transfer
  • Tenotomy

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine


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