Differential expression of synaptosome-associated protein 25 kDa [SNAP- 25] in hippocampi of neonatal mice following exposure to human influenza virus in utero

S. Hossein Fatemi*, Robert Sidwell, David Kist, Pervez Akhter, Herbert Y. Meltzer, Kevin Bailey, Paul Thuras, Jerry Sedgwick

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

We investigated the role of maternal exposure to human influenza virus [HI] in C57BL/6 mice on day 9 of pregnancy on the hippocampal expression of SNAP-25 in postnatal day 0 neonates, and compared them to sham-infected pups. The expression of SNAP-25 in infected neonates varied along the septotemporal axis of hippocampus and in various anatomic layers. Quantitative densitometric analysis of specific immunogold silver-enhanced SNAP-25 immunoreactivity [IR] showed increases of 40-347% over control in all septal- dorsal hippocampal layers except for the subplate layer. In mid septo- temporal hippocampus, SNAP-25 IR increased by 10-114% over control in all layers, except for the hippocampal plate, but the extent of this increase was smaller than in the dorsal-septal area. Finally, in temporal-ventral levels, SNAP-25 expression was reduced in all infected layers by 21-33% below control except for mild increases of 8.8 and 10% in subplate and hippocampal plate layers. Additionally, the infected SNAP-25 maximal density bin shifted to lower values dorsally and to higher values medially, with ventral maximal bins remaining unchanged when compared to controls. The differential expression of SNAP-25 in the hippocampi of infected neonates indicates a variable degree of vulnerability across the septo-temporal axis of hippocampus. It is surmised that while viral infection may induce excitotoxicity in the ventral hippocampus, it may cause reactive synapto- genesis in the medial and dorsal sectors of the developing brains of postnatal day 0 neonates.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalBrain research
Volume800
Issue number1
DOIs
StatePublished - Jul 27 1998

Keywords

  • Autism
  • Hippocampus
  • Human influenza virus
  • Mice
  • SNAP-25
  • Schizophrenia
  • Second trimester of pregnancy

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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