Differential expression of the coxsackievirus and adenovirus receptor regulates adenovirus infection of the placenta

H. Koi, J. Zhang, A. Makrigiannakis, S. Getsios, C. D. MacCalman, G. S. Kopf, J. F. Strauss, S. Parry*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

The molecular mechanisms and pathologic significance of placental viral infections are poorly understood. We investigated factors that regulate placental infection by adenovirus, which is the most common viral pathogen identified in fetal samples from abnormal pregnancies (i.e., fetal growth restriction, oligohydramnios, and nonimmune fetal hydrops). We also determined the pathologic significance of placental adenovirus infection. Northern hybridization, flow cytometry, and immunostaining revealed that placental expression of the coxsackievirus and adenovirus receptor (CAR) varied with gestational age and trophoblast phenotype. The CAR was continuously expressed in invasive or extravillous trophoblast cells but not in villous trophoblast cells. We postulate that the villous syncytiotrophoblast, which does not express CAR and is resistant to adenovirus infection, limits the transplacental transmission of viral pathogens, including adenovirus. Conversely, extravillous trophoblast cells underwent apoptosis when infected by adenovirus in the presence of decidual lymphocytes (which simulated the maternal immune response to viral infection). Thus, adenovirus infection and/or the maternal immune response to adenovirus infection induced the death of placental cell types that expressed CAR. Consequently, we speculate that adenovirus infection of extravillous trophoblast cells may negatively impact the process of placental invasion and predispose the mother and fetus to adverse reproductive outcomes that result from placental dysfunction.

Original languageEnglish (US)
Pages (from-to)1001-1009
Number of pages9
JournalBiology of reproduction
Volume64
Issue number3
DOIs
StatePublished - 2001

Keywords

  • Adenovirus
  • Apoptosis
  • Placenta
  • Pregnancy
  • Trophoblast

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

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