Differential growth factor induction and modulation of human gastric epithelial regeneration

Marie Pier Tétreault, Pierre Chailler, Nathalie Rivard, Daniel Ménard*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


While several autocrine/paracrine growth factors (GFs) can all stimulate epithelial regeneration in experimentally wounded primary gastric cultures, clinical relevance for their non-redundant cooperative actions in human gastric ulcer healing is suggested by the sequential pattern of GF gene induction in vivo. Using new HGE cell lines able to form a coherent monolayer with tight junctions as well as using primary human gastric epithelial cultures, we show that EGF, TGFα, HGF and IGFs accelerate epithelial restitution upon wounding, independently of the TGFβ pathway (as opposed to intestinal cells). However, they differently modulate cell behavior: TGFα exerts strong effects (even more than EGF) on cytoplasmic spreading and non-oriented protruding activity of bordering cells whereas HGF preferentially coordinates single lamella formation, cell elongation and migration into the wound. IGF-I and IGF-II rather induce the alignment of bordering cells and maintain a compact monolayer front. The number of mitotic cells maximally increases with EGF, followed by TGFα and IGF-I,-II. The current study demonstrates that GFs differentially regulate the regeneration of human gastric epithelial cells through specific modulation of cell shape adaptation, migration and proliferation, further stressing that a coordination of GF activities would be necessary for the normal progression of post-wounding epithelial repair.

Original languageEnglish (US)
Pages (from-to)285-297
Number of pages13
JournalExperimental Cell Research
Issue number1
StatePublished - May 15 2005


  • Cell migration
  • Cell spreading
  • Epithelial restitution
  • Human stomach
  • Ulcer healing

ASJC Scopus subject areas

  • Cell Biology


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