TY - JOUR
T1 - Differential HLA allele frequency in Mycobacterium africanum vs Mycobacterium tuberculosis in Mali
AU - Kone, Amadou
AU - Diarra, Bassirou
AU - Cohen, Keira
AU - Diabate, Seydou
AU - Kone, Bourahima
AU - Diakite, Mahamane T.
AU - Diarra, Hawa
AU - Sanogo, Moumine
AU - Togo, Antieme C.G.
AU - Sarro, Yeya dit Sadio
AU - Baya, Bocar
AU - Coulibaly, Nadie
AU - Kodio, Ousmane
AU - Achenbach, Chad J.
AU - Murphy, Robert L.
AU - Holl, Jane L.
AU - Siddiqui, Sophia
AU - Doumbia, Seydou
AU - Bishai, William R.
AU - Diallo, Souleymane
AU - Maiga, Mamoudou
N1 - Funding Information:
The authors would like to acknowledge all SEREFO/UCRC staff, laboratory and clinical who contributed to patient recruitment, sample processing and data collection. We are grateful to all the volunteers who were willing to participate into the study. This work was supported by the National Institutes of Health (R01AI110386, D43TW010350 and D71TW010428).
Publisher Copyright:
© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
PY - 2019/1
Y1 - 2019/1
N2 - Tuberculosis (TB) is caused by Mycobacterium tuberculosis complex (MTBC), however, the distribution and frequency of MTBC lineages and sublineages vary in different parts of the globe. Mycobacterium africanum, a member of MTBC is responsible for a large percentage of TB cases in West Africa, however, it is rarely identified outside of this part of the World. Whether or not differential HLA polymorphism (an important host factor) is contributing to the geographic restriction of M. africanum to West Africa is unknown. Here, we conducted a cohort study in Mali of newly diagnosed individuals with active pulmonary TB and normal healthy controls. The MTBC isolates were spoligotyped to determine the TB study groups (M. tuberculosis sensu stricto LAM10 and M. africanum), and HLA typing was performed on peripheral blood. Unlike previous reports on other populations, we found that HLA class-I alleles were significantly associated with active TB disease in this population. HLA-B alleles (B*07:02, B*08:01, B*14:02, B*15:03, B*15:10, B*18:01, B*42:01, B*42:02, B*51:01 and B*81:01) were significantly associated with M. africanum (40%-45%) and M. tuberculosis (75%) compared with healthy controls. Many HLA-A alleles (A*02:05, A*34:02, A*66:01 and A*68:02) were also associated with both TB groups (65%-70%). However, many class II HLA-DR variants were found to be associated with M. tuberculosis but not M. africanum with the exception of the DRB1*03:01, which was associated with both groups. The differential HLA distribution observed in this study might be at least partially responsible for the geographical restriction of M. africanum infections to West Africa.
AB - Tuberculosis (TB) is caused by Mycobacterium tuberculosis complex (MTBC), however, the distribution and frequency of MTBC lineages and sublineages vary in different parts of the globe. Mycobacterium africanum, a member of MTBC is responsible for a large percentage of TB cases in West Africa, however, it is rarely identified outside of this part of the World. Whether or not differential HLA polymorphism (an important host factor) is contributing to the geographic restriction of M. africanum to West Africa is unknown. Here, we conducted a cohort study in Mali of newly diagnosed individuals with active pulmonary TB and normal healthy controls. The MTBC isolates were spoligotyped to determine the TB study groups (M. tuberculosis sensu stricto LAM10 and M. africanum), and HLA typing was performed on peripheral blood. Unlike previous reports on other populations, we found that HLA class-I alleles were significantly associated with active TB disease in this population. HLA-B alleles (B*07:02, B*08:01, B*14:02, B*15:03, B*15:10, B*18:01, B*42:01, B*42:02, B*51:01 and B*81:01) were significantly associated with M. africanum (40%-45%) and M. tuberculosis (75%) compared with healthy controls. Many HLA-A alleles (A*02:05, A*34:02, A*66:01 and A*68:02) were also associated with both TB groups (65%-70%). However, many class II HLA-DR variants were found to be associated with M. tuberculosis but not M. africanum with the exception of the DRB1*03:01, which was associated with both groups. The differential HLA distribution observed in this study might be at least partially responsible for the geographical restriction of M. africanum infections to West Africa.
KW - HLA
KW - M. africanum
KW - Mali
KW - West Africa
KW - tuberculosis
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U2 - 10.1111/tan.13448
DO - 10.1111/tan.13448
M3 - Article
C2 - 30516034
AN - SCOPUS:85059354474
SN - 2059-2302
VL - 93
SP - 24
EP - 31
JO - HLA
JF - HLA
IS - 1
ER -
