Differential Intrasplenic Migration of Dendritic Cell Subsets Tailors Adaptive Immunity

Samuele Calabro, Dong Liu, Antonia Gallman, Manuela Sales L. Nascimento, Zizi Yu, Ting ting Zhang, Pei Chen, Biyan Zhang, Lan Xu, Uthaman Gowthaman, Jayendra Kumar Krishnaswamy, Ann M. Haberman, Adam Williams*, Stephanie C. Eisenbarth

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

87 Scopus citations

Abstract

Evidence suggests that distinct splenic dendritic cell (DC) subsets activate either CD4+ or CD8+ T cells in vivo. This bias has been partially ascribed to differential antigen presentation; however, all DC subsets can activate both T cell lineages in vitro. Therefore, we tested whether the organization of DC and T cell subsets in the spleen dictated this preference. We discovered that CD4+ and CD8+ T cells segregated within splenic T cell zones prior to immunization. After intravenous immunization, the two major conventional DC populations, distinguished by 33D1 and XCR1 staining, migrated into separate regions of the T cell zone: 33D1+ DCs migrated into the CD4+ T cell area, whereas XCR1+ DCs migrated into the CD8+ T cell area. Thus, the post-immunization location of each DC subset correlated with the T cell lineage it preferentially primes. Preventing this co-localization selectively impaired either CD4+ or CD8+ T cell immunity to blood-borne antigens.

Original languageEnglish (US)
Pages (from-to)2472-2485
Number of pages14
JournalCell reports
Volume16
Issue number9
DOIs
StatePublished - Aug 30 2016
Externally publishedYes

Keywords

  • CCR7
  • T cell
  • dendritic cell
  • red blood cell alloimmunization
  • spleen

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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