Abstract
An accurate biomarker for detection of ovarian cancer may reduce cancer-related mortality. Using a previously developed microarray-based technique, we evaluated differences in ONA methylation profiles in a panel of 56 genes using sections of serous papillary adenocarcinomas and uninvolved ovaries (n = 30) from women in a high-risk group. Methylation profiles were also generated for circulating ONA from blood of patients (n = 33) and healthy controls (n = 33). Using the most differentially methylated genes for naïve Bayesian analysis, we identified ten of these profiles as potentially informative in tissues. Various combinations of these genes produced 69% sensitivity and 70% specificity for cancer detection as estimated under a stratified, fivefold cross-validation protocol. In plasma, five genes were identified as informative; their combination had 85% sensitivity and 61% specificity for cancer detection. These results suggest that differential methylation profiling in heterogeneous samples has the potential to identify components of a composite biomarker that may detect ovarian cancer in blood with significant accuracy.
Original language | English (US) |
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Pages (from-to) | 60-65 |
Number of pages | 6 |
Journal | Journal of Molecular Diagnostics |
Volume | 11 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2009 |
Funding
Supported by Illinois Department of Public Health, Penny Severns Breast, Cervical, and Ovarian Cancer Research Fund; the Marsha Rivkin Center for Ovarian Cancer Research, Babs Fisher Pilot Study Award to V.V.L. ; SPORE P-50 CA 83638 and 5U01 CA113916 to A.K.G.
ASJC Scopus subject areas
- Molecular Medicine
- Pathology and Forensic Medicine