Differential modulation of respiratory neuronal discharge patterns by GABAA receptor and apamin-sensitive K+ channel antagonism

V. Tonkovic-Capin, A. G. Stucke, E. A. Stuth, M. Tonkovic-Capin, M. Krolo, F. A. Hopp, D. R. Mccrimmon, E. J. Zuperku*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The discharge patterns of respiratory neurons of the caudal ventral respiratory group (cVRG) appear to be subject to potent GABAergic gain modulation. Local application of the GABAA receptor antagonist bicuculline methochloride amplifies the underlying discharge frequency (Fn) patterns mediated by endogenous excitatory and inhibitory synaptic inputs, Gain modulation can also be produced by alterations in the amplitude of spike afterhyperpolarizations (AHPs) mediated by apamin-sensitive small-conductance Ca2+-activated K+ (SK) channels. Since methyl derivatives of bicuculline (BICm) also have been shown to reduce the amplitude of AHPs, in vitro, it is possible that the BICm-induced gain modulation is due to a block of SK channels. The purpose of these studies was to determine the mechanisms by which BICm produces gain modulation and to characterize the influence of SK channels in the control of respiratory neuron discharge. Six protocols were used in this in vivo study of cVRG inspiratory (1) and expiratory (E) neurons in decerebrate, paralyzed, ventilated dogs. The protocols included characterizations of the neuronal responses to 1) BICm and apamin on the same neuron, 2) BICm during maximum apamin-induced block of AHPs, 3) apamin during maximum BICm-induced gain modulatory responses, 4) the specific GABAA receptor antagonist, (+)β-hydrastine, 5) the specific GABAA receptor agonist, muscimol, and 6) the GABA uptake inhibitor, nipecotic acid, For protocols 3, 5, and 6, only E neurons were studied. Four-barrel micropipettes were used for extracellular single neuron recording and pressure ejection of drugs. Cycle-triggered histograms were used to quantify the Fn patterns and to determine the drug-induced changes in the gain (slope) and offset of the Fn patterns. Compared to apamin at maximum effective dose rates, BICm produced a 2.1-fold greater increase in peak Fn and a 3.1-fold greater increase in average Fn BICm and apamin produced similar increases in gain, but the offsets due to apamin were more negative. The responses to hydrastine were similar to BICm. During maximum apamin block, BICm produced an additional 112 ± 22% increase in peak Fn. Conversely, apamin produced an additional 176 ± 74% increase in peak Fn during the maximum BICm-induced response. Muscimol and nipecotic acid both decreased the gain and offset of the discharge patterns. Taken together, these results suggest that the gain modulatory effect of BICm is due to a reduction of GABAA-ergic shunting inhibition rather than a reduction in AHPs by block of SK channels in canine cVRG neurons.

Original languageEnglish (US)
Pages (from-to)2363-2373
Number of pages11
JournalJournal of neurophysiology
Volume86
Issue number5
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

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