Differential neuronal and glial behavior on flat and micro patterned chitosan films

Marta Mattotti, Zaida Alvarez Pinto, Luis Delgado, Miguel A. Mateos-Timoneda, Conrado Aparicio, Josep A. Planell, Soledad Alcántara, Elisabeth Engel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Chitosan is a biodegradable natural polysaccharide that has been widely studied for regenerative purposes in the central nervous system. In this study we assessed the in vitro glial and neuronal cells response to chitosan either flat or patterned with grooves in the micrometric range. Chitosan demonstrated to be a good substrate for the attachment and growth of both neurons and glial cells. Chitosan micropatterns promoted glial cell maturation, suggesting astroglial activation. Nevertheless, those mature/reactive glial cells were permissive for axonal growth. Axons aligned and organized along the patterned grooves and the size of the linear topographic patterns is also affecting neurite and cell response. Patterns with 10 μm width induced fasciculation of axons, which can be useful for CNS tissue engineering substrates when precise orientation of the axonal outgrowth is desired.

Original languageEnglish (US)
Pages (from-to)569-577
Number of pages9
JournalColloids and Surfaces B: Biointerfaces
Volume158
DOIs
StatePublished - Oct 1 2017

Keywords

  • Brain
  • Chitosan
  • Glia
  • Micropattern
  • Neuron

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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