Differential precipitation and zinc chelate chromatography purification of biologically active HTLV-I Tax1 expressed in E. coli

Paul F. Lindholm*, Susan J. Marriott, Scott D. Gitlin, John N. Brady

*Corresponding author for this work

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A protocol which involves sequential ammonium sulfate precipitation and zinc chelate chromatography to purify the HTLV-I Tax1 protein expressed in E. coli is described. The final Tax1 product is greater than 90% pure and the yield is approximately 1 mg per liter of liquid culture. The purified Tax1 protein is biologically active in indirect in vitro DNA binding assays and cellular NF-kB induction experiments.

Original languageEnglish (US)
Pages (from-to)233-241
Number of pages9
JournalJournal of Biochemical and Biophysical Methods
Volume22
Issue number3
DOIs
StatePublished - Jan 1 1991

Keywords

  • HLTV-I Tax
  • Purification

ASJC Scopus subject areas

  • Biophysics

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