Differential precipitation and zinc chelate chromatography purification of biologically active HTLV-I Tax1 expressed in E. coli

Paul F. Lindholm; Susan J. Marriott; Scott D. Gitlin; John N. Brady

doi:10.1016/0165-022X(91)90071-4

Differential precipitation and zinc chelate chromatography purification of biologically active HTLV-I Tax₁ expressed in E. coli

Paul F. Lindholm^*, Susan J. Marriott, Scott D. Gitlin, John N. Brady

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A protocol which involves sequential ammonium sulfate precipitation and zinc chelate chromatography to purify the HTLV-I Tax₁ protein expressed in E. coli is described. The final Tax₁ product is greater than 90% pure and the yield is approximately 1 mg per liter of liquid culture. The purified Tax₁ protein is biologically active in indirect in vitro DNA binding assays and cellular NF-kB induction experiments.

Original language	English (US)
Pages (from-to)	233-241
Number of pages	9
Journal	Journal of biochemical and biophysical methods
Volume	22
Issue number	3
DOIs	https://doi.org/10.1016/0165-022X(91)90071-4
State	Published - Apr 1991

Keywords

HLTV-I Tax
Purification

ASJC Scopus subject areas

Biophysics
Biochemistry

Access to Document

10.1016/0165-022X(91)90071-4

Cite this

@article{049364fc36324ec19564c5cc83e883ca,

title = "Differential precipitation and zinc chelate chromatography purification of biologically active HTLV-I Tax1 expressed in E. coli",

abstract = "A protocol which involves sequential ammonium sulfate precipitation and zinc chelate chromatography to purify the HTLV-I Tax1 protein expressed in E. coli is described. The final Tax1 product is greater than 90% pure and the yield is approximately 1 mg per liter of liquid culture. The purified Tax1 protein is biologically active in indirect in vitro DNA binding assays and cellular NF-kB induction experiments.",

keywords = "HLTV-I Tax, Purification",

author = "Lindholm, {Paul F.} and Marriott, {Susan J.} and Gitlin, {Scott D.} and Brady, {John N.}",

year = "1991",

month = apr,

doi = "10.1016/0165-022X(91)90071-4",

language = "English (US)",

volume = "22",

pages = "233--241",

journal = "Journal of Proteomics",

issn = "1874-3919",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Differential precipitation and zinc chelate chromatography purification of biologically active HTLV-I Tax1 expressed in E. coli

AU - Lindholm, Paul F.

AU - Marriott, Susan J.

AU - Gitlin, Scott D.

AU - Brady, John N.

PY - 1991/4

Y1 - 1991/4

N2 - A protocol which involves sequential ammonium sulfate precipitation and zinc chelate chromatography to purify the HTLV-I Tax1 protein expressed in E. coli is described. The final Tax1 product is greater than 90% pure and the yield is approximately 1 mg per liter of liquid culture. The purified Tax1 protein is biologically active in indirect in vitro DNA binding assays and cellular NF-kB induction experiments.

AB - A protocol which involves sequential ammonium sulfate precipitation and zinc chelate chromatography to purify the HTLV-I Tax1 protein expressed in E. coli is described. The final Tax1 product is greater than 90% pure and the yield is approximately 1 mg per liter of liquid culture. The purified Tax1 protein is biologically active in indirect in vitro DNA binding assays and cellular NF-kB induction experiments.

KW - HLTV-I Tax

KW - Purification

UR - http://www.scopus.com/inward/record.url?scp=0025975981&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025975981&partnerID=8YFLogxK

U2 - 10.1016/0165-022X(91)90071-4

DO - 10.1016/0165-022X(91)90071-4

M3 - Article

C2 - 1865054

AN - SCOPUS:0025975981

VL - 22

SP - 233

EP - 241

JO - Journal of Proteomics

JF - Journal of Proteomics

SN - 1874-3919

IS - 3

ER -