Abstract
A protocol which involves sequential ammonium sulfate precipitation and zinc chelate chromatography to purify the HTLV-I Tax1 protein expressed in E. coli is described. The final Tax1 product is greater than 90% pure and the yield is approximately 1 mg per liter of liquid culture. The purified Tax1 protein is biologically active in indirect in vitro DNA binding assays and cellular NF-kB induction experiments.
Original language | English (US) |
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Pages (from-to) | 233-241 |
Number of pages | 9 |
Journal | Journal of biochemical and biophysical methods |
Volume | 22 |
Issue number | 3 |
DOIs | |
State | Published - Apr 1991 |
Keywords
- HLTV-I Tax
- Purification
ASJC Scopus subject areas
- Biophysics
- Biochemistry