Differential precipitation and zinc chelate chromatography purification of biologically active HTLV-I Tax1 expressed in E. coli

Paul F. Lindholm; Susan J. Marriott; Scott D. Gitlin; John N. Brady

doi:10.1016/0165-022X(91)90071-4

Differential precipitation and zinc chelate chromatography purification of biologically active HTLV-I Tax₁ expressed in E. coli

Paul F. Lindholm^*, Susan J. Marriott, Scott D. Gitlin, John N. Brady

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article

14 Scopus citations

Abstract

A protocol which involves sequential ammonium sulfate precipitation and zinc chelate chromatography to purify the HTLV-I Tax₁ protein expressed in E. coli is described. The final Tax₁ product is greater than 90% pure and the yield is approximately 1 mg per liter of liquid culture. The purified Tax₁ protein is biologically active in indirect in vitro DNA binding assays and cellular NF-kB induction experiments.

Original language	English (US)
Pages (from-to)	233-241
Number of pages	9
Journal	Journal of Biochemical and Biophysical Methods
Volume	22
Issue number	3
DOIs	https://doi.org/10.1016/0165-022X(91)90071-4
State	Published - Jan 1 1991

Keywords

HLTV-I Tax
Purification

ASJC Scopus subject areas

Biophysics

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10.1016/0165-022X(91)90071-4

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abstract = "A protocol which involves sequential ammonium sulfate precipitation and zinc chelate chromatography to purify the HTLV-I Tax1 protein expressed in E. coli is described. The final Tax1 product is greater than 90% pure and the yield is approximately 1 mg per liter of liquid culture. The purified Tax1 protein is biologically active in indirect in vitro DNA binding assays and cellular NF-kB induction experiments.",

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AU - Gitlin, Scott D.

AU - Brady, John N.

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