Differential protein expression in the cytosol fraction of an MCF-7 breast cancer cell line selected for resistance toward melphalan

Yetrib Hathout*, Kelly Riordan, Marion Gehrmann, Catherine Fenselau

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Analysis of differential protein expression in the cytosol of melphalan-resistant and -susceptible MCF-7 cell lines has been carried out using a combination of two-dimensional gel electrophoresis, mass spectrometry, and bioinformatics. Comparison of multiple digitized gel arrays detected several spots as candidates for differentially expressed proteins in melphalan-resistant MCF-7 cells. The up-regulated proteins included retinoic acid binding protein II, an isoform of the macrophage migration inhibition factor, and other unidentified proteins. The down-regulated proteins included calreticulin, cyclophin A, and an isoform of the 27 kD heat shock protein. Correlation of the differential expression of some of the proteins with acquired resistance of MCF7 cells to melphalan is discussed.

Original languageEnglish (US)
Pages (from-to)435-442
Number of pages8
JournalJournal of Proteome Research
Volume1
Issue number5
DOIs
StatePublished - Sep 2002
Externally publishedYes

Keywords

  • 27 kD heat shock protein
  • Calreticulin
  • Chemoresistance
  • Cofilin
  • Cyclophilin
  • Macrophage migration inhibition factor
  • MCF-7
  • Melphalan
  • Post-translational modification
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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