Differential regulation of human basophil and lung mast cell function by adenosine

P. T. Peachell, L. M. Lichtenstein, R. P. Schleimer*

*Corresponding author for this work

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

Adenosine was found to modulate the activity of the human basophil and lung mast cell (HLMC) differently. In the basophil, adenosine inhibited the anti-IgE stimulated release of histamine and leukotriene C4 (LTC4) and increased total cell cyclic AMP (cAMP) levels. Substituted adenosine analogs had a rank order potency of: N-ethylcarboxamideadenosine (NECA) > 2-chloroadenosine > R-phenylisopropyladenosine for the inhibition of immunoglobulin E-triggered mediator release from the basophil and increases in cAMP levels. The adenosine receptor antagonist, 8-phenyltheophylline, antagonized both the NECA-induced inhibition of mediator release and elevations in cyclic nucleotide. The purinergic transport inhibitor, dipyridamole, reversed the inhibition by adenosine of histamine release but not LTC4 generation, suggesting that these two actions are mechanistically separable. Dipyridamole failed to modify the adenosine-induced elevation in cAMP. In contrast to the findings in the basophil, the response to adenosine in the HLMC was biphasic in nature. Thus, at low concentrations of the nucleoside, adenosine potentiated the release of histamine and LTC4 from immunologically activated HLMC, whereas at higher concentrations a counteractive inhibitory process was observed. Analogs of adenosine had the same effects on HLMC; NECA was more potent than R-phenylisopropyladenosine for both the potentiating and inhibitory components of the biphasic response. Low concentrations of adenosine analogs, which potentiated secretion, initiated modest elevations in cAMP levels, whereas higher concentrations, which inhibited secretion, significantly augmented cAMP levels. Although R-phenylisopropyladenosine was almost as potent as NECA at elevating cAMP in HLMC, it was not as efficacious. The NECA-induced modulation of HLMC mediator release and elevations in cAMP were antagonized by 8-phenyltheophylline. In total, these results suggest that the basophil possesses an alpha2 receptor but that adenosine, as well as interacting with this receptor, also exerts intracellular, cAMP-independent effects, which are involved in countering the release of histamine but not LTC4. In the HLMC, rationalizing the experimental data to adenosine receptor subtypes proves more problematical. It is suggested that the inhibitory arm of the biphasic response is mediated through activation of an adenylate cyclase-coupled atypical alpha2 receptor. Either the same receptor coupled to an effector system other than adenylate cyclase or a distinct and previously uncharacterized receptor may mediate the enhancement of secretion.

Original languageEnglish (US)
Pages (from-to)717-726
Number of pages10
JournalJournal of Pharmacology and Experimental Therapeutics
Volume256
Issue number2
StatePublished - 1991

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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