Differential requirements for clathrin endocytic pathway components in cellular entry by Ebola and Marburg glycoprotein pseudovirions

Suchita Bhattacharyya, Thomas J. Hope*, John A.T. Young

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Clathrin-mediated endocytosis was previously implicated as one of the cellular pathways involved in filoviral glycoprotein mediated viral entry into target cells. Here we have further dissected the requirements for different components of this pathway in Ebola versus Marburg virus glycoprotein (GP) mediated viral infection. Although a number of these components were involved in both cases; Ebola GP-dependent viral entry specifically required the cargo recognition proteins Eps15 and DAB2 as well as the clathrin adaptor protein AP-2. In contrast, Marburg GP-mediated infection was independent of these three proteins and instead required beta-arrestin 1 (ARRB1). These findings have revealed an unexpected difference between the clathrin pathway requirements for Ebola GP versus Marburg GP pseudovirion infection. Anthrax toxin also uses a clathrin-, and ARRB1-dependent pathway for cellular entry, indicating that the mechanism used by Marburg GP pseudovirions may be more generally important for pathogen entry.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalVirology
Volume419
Issue number1
DOIs
StatePublished - Oct 10 2011

Keywords

  • AP-1
  • AP-2
  • ARRB1
  • Clathrin-mediated endocytosis
  • DAB2
  • Entry
  • Eps15
  • Filoviral GP
  • Pseudovirions

ASJC Scopus subject areas

  • Virology

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