Abstract
Clathrin-mediated endocytosis was previously implicated as one of the cellular pathways involved in filoviral glycoprotein mediated viral entry into target cells. Here we have further dissected the requirements for different components of this pathway in Ebola versus Marburg virus glycoprotein (GP) mediated viral infection. Although a number of these components were involved in both cases; Ebola GP-dependent viral entry specifically required the cargo recognition proteins Eps15 and DAB2 as well as the clathrin adaptor protein AP-2. In contrast, Marburg GP-mediated infection was independent of these three proteins and instead required beta-arrestin 1 (ARRB1). These findings have revealed an unexpected difference between the clathrin pathway requirements for Ebola GP versus Marburg GP pseudovirion infection. Anthrax toxin also uses a clathrin-, and ARRB1-dependent pathway for cellular entry, indicating that the mechanism used by Marburg GP pseudovirions may be more generally important for pathogen entry.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1-9 |
| Number of pages | 9 |
| Journal | Virology |
| Volume | 419 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 10 2011 |
Funding
This work was supported by a National Institutes of Health (NIH) grant AI052051 to T.J.H and funding from the Nomis Foundation , the James B. Pendleton Charitable Trust and the Rodney Dean and Cornelia Hayes Mackey Endowment Fund to J.A.T.Y. T.J.H is also an Elizabeth Glaser Scientist.
Keywords
- AP-1
- AP-2
- ARRB1
- Clathrin-mediated endocytosis
- DAB2
- Entry
- Eps15
- Filoviral GP
- Pseudovirions
ASJC Scopus subject areas
- Virology
