Differential roles of 2, 6, and 8 carbon ceramides on the modulation of gap junctional communication and apoptosis during carcinogenesis

Brad L. Upham; Tyler R. Koski; Alisa M. Rummel; Melinda R. Wilson; Anelia Horvath; James E. Trosko

doi:10.1016/S0304-3835(02)00620-1

Differential roles of 2, 6, and 8 carbon ceramides on the modulation of gap junctional communication and apoptosis during carcinogenesis

Brad L. Upham^*, Tyler R. Koski, Alisa M. Rummel, Melinda R. Wilson, Anelia Horvath, James E. Trosko

^*Corresponding author for this work

Neurological Surgery

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The inhibition of apoptosis and gap junctional intercellular communication (GJIC) has been implicated in tumor promotion. Ionizing radiation and oxidative toxicants activate sphingomyelinases resulting in the release of ceramides that control cell proliferation and apoptosis. A rat liver epithelial cell line treated with ceramides containing a 6 (C6) or 8 (C8) carbon acyl-group were potent inhibitors of GJIC and apoptosis, whereas a C2-ceramide was only a weak inhibitor of GJIC and strong inducer of apoptosis. Apoptosis induced by either serum deprivation or C2-ceramide was inhibited by the GJIC inhibitory C8-ceramide. In conclusion, these results suggest that a chronic release of ceramides with acyl groups larger than C6 might act as tumor promoters.

Original language	English (US)
Pages (from-to)	27-34
Number of pages	8
Journal	Cancer Letters
Volume	191
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3835(02)00620-1
State	Published - Feb 28 2003

Funding

We thank Mr Jacob Scott for his technical assistance in the Western blot analysis of Cx43. This research was supported by the National Institute of Environmental Health Science Superfund (grant #P42 ES04911-07) to JET.

Keywords

Apoptosis
Ceramide
Gap junctional intercellular communication
Signal transduction
Tumor promotion

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0304-3835(02)00620-1

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title = "Differential roles of 2, 6, and 8 carbon ceramides on the modulation of gap junctional communication and apoptosis during carcinogenesis",

abstract = "The inhibition of apoptosis and gap junctional intercellular communication (GJIC) has been implicated in tumor promotion. Ionizing radiation and oxidative toxicants activate sphingomyelinases resulting in the release of ceramides that control cell proliferation and apoptosis. A rat liver epithelial cell line treated with ceramides containing a 6 (C6) or 8 (C8) carbon acyl-group were potent inhibitors of GJIC and apoptosis, whereas a C2-ceramide was only a weak inhibitor of GJIC and strong inducer of apoptosis. Apoptosis induced by either serum deprivation or C2-ceramide was inhibited by the GJIC inhibitory C8-ceramide. In conclusion, these results suggest that a chronic release of ceramides with acyl groups larger than C6 might act as tumor promoters.",

keywords = "Apoptosis, Ceramide, Gap junctional intercellular communication, Signal transduction, Tumor promotion",

author = "Upham, {Brad L.} and Koski, {Tyler R.} and Rummel, {Alisa M.} and Wilson, {Melinda R.} and Anelia Horvath and Trosko, {James E.}",

note = "Funding Information: We thank Mr Jacob Scott for his technical assistance in the Western blot analysis of Cx43. This research was supported by the National Institute of Environmental Health Science Superfund (grant #P42 ES04911-07) to JET. ",

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doi = "10.1016/S0304-3835(02)00620-1",

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T1 - Differential roles of 2, 6, and 8 carbon ceramides on the modulation of gap junctional communication and apoptosis during carcinogenesis

AU - Upham, Brad L.

AU - Koski, Tyler R.

AU - Rummel, Alisa M.

AU - Wilson, Melinda R.

AU - Horvath, Anelia

AU - Trosko, James E.

N1 - Funding Information: We thank Mr Jacob Scott for his technical assistance in the Western blot analysis of Cx43. This research was supported by the National Institute of Environmental Health Science Superfund (grant #P42 ES04911-07) to JET.

PY - 2003/2/28

Y1 - 2003/2/28

N2 - The inhibition of apoptosis and gap junctional intercellular communication (GJIC) has been implicated in tumor promotion. Ionizing radiation and oxidative toxicants activate sphingomyelinases resulting in the release of ceramides that control cell proliferation and apoptosis. A rat liver epithelial cell line treated with ceramides containing a 6 (C6) or 8 (C8) carbon acyl-group were potent inhibitors of GJIC and apoptosis, whereas a C2-ceramide was only a weak inhibitor of GJIC and strong inducer of apoptosis. Apoptosis induced by either serum deprivation or C2-ceramide was inhibited by the GJIC inhibitory C8-ceramide. In conclusion, these results suggest that a chronic release of ceramides with acyl groups larger than C6 might act as tumor promoters.

AB - The inhibition of apoptosis and gap junctional intercellular communication (GJIC) has been implicated in tumor promotion. Ionizing radiation and oxidative toxicants activate sphingomyelinases resulting in the release of ceramides that control cell proliferation and apoptosis. A rat liver epithelial cell line treated with ceramides containing a 6 (C6) or 8 (C8) carbon acyl-group were potent inhibitors of GJIC and apoptosis, whereas a C2-ceramide was only a weak inhibitor of GJIC and strong inducer of apoptosis. Apoptosis induced by either serum deprivation or C2-ceramide was inhibited by the GJIC inhibitory C8-ceramide. In conclusion, these results suggest that a chronic release of ceramides with acyl groups larger than C6 might act as tumor promoters.

KW - Apoptosis

KW - Ceramide

KW - Gap junctional intercellular communication

KW - Signal transduction

KW - Tumor promotion

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DO - 10.1016/S0304-3835(02)00620-1

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SN - 0304-3835

VL - 191

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JO - Cancer Letters

JF - Cancer Letters

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ER -

Differential roles of 2, 6, and 8 carbon ceramides on the modulation of gap junctional communication and apoptosis during carcinogenesis

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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