Differential susceptibility of midbrain and spinal cord patterning to floor plate defects in the talpid2 mutant

Seema Agarwala*, Galina V. Aglyamova, Amanda K Marma, John F. Fallon, Clifton W. Ragsdale

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The chick talpid2 mutant displays polydactylous digits attributed to defects of the Hedgehog (HH) signaling pathway. We examined the talpid2 neural tube and show that patterning defects in the spinal cord and the midbrain are distinct from each other and from the limb. Unlike the Sonic Hedgehog (SHH) source in the limb, the SHH-rich floor plate (FP) is reduced in the talpid2 midbrain. This is accompanied by a severe depletion of medial cell populations that encounter high concentrations of SHH, an expansion of lateral cell populations that experience low concentrations of SHH and a broad deregulation of HH's principal effectors (PTC1, GLI1, GLI2, GLI3). Together with the failure of SHH misexpression to rescue the talpid 2 phenotype, these results suggest that talpid2 is likely to have a tissue-autonomous, bidirectional (positive and negative) role in HH signaling that cannot be attributed to the altered expression of several newly cloned HH pathway genes (SUFU, DZIP1, DISP1, BTRC). Strikingly, FP defects in the spinal cord are accompanied by relatively normal patterning in the talpid2 mutant. We propose that this differential FP dependence may be due to the prolonged apposition of the notochord to the spinal cord, but not the midbrain during development.

Original languageEnglish (US)
Pages (from-to)206-220
Number of pages15
JournalDevelopmental Biology
Volume288
Issue number1
DOIs
StatePublished - Dec 1 2005

Keywords

  • Dopaminergic neurons
  • Floor plate
  • Midbrain
  • Motor neurons
  • Notochord
  • Sonic Hedgehog
  • Spinal cord

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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